By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Enzymes and Coenzymes have been published. According to news reporting originating from Andhra Pradesh, India, by NewsRx correspondents, research stated, "Aspergillus terreus MTCC 11096 isolated from the soils of agricultural fields cultivating sweet sorghum was previously identified to produce feruloyl esterases (FAEs). The enzymes responsible for feruloyl esterase activity were purified to homogeneity and named as AtFAE-1, AtFAE-2, and AtFAE-3."
Our news editors obtained a quote from the research from Acharya Nagarjuna University, "The enzymes were monomeric having molecular masses of 74, 23 and 36 kDa, respectively. Active protein bands were identified by a developed pH-dependent zymogram on native PAGE. The three enzymes exhibited variation in pH tolerance ranging between pH 5-8 and thermostability of up to 55 degrees C. Inhibition studies revealed that the serine residue was essential for feruloyl esterase activity; moreover aspartyl and glutamyl residues are not totally involved at the active site. Metal ions such as Ca2+, K+, and Mg2+ stabilized the enzyme activity for all three FAEs. Kinetic data indicated that all three enzymes showed catalytic efficiencies (k(cat)/K-m) against different synthesized alkyl and aryl esters indicating their broad substrate specificity. The peptide mass fingerprinting by MALDI/TOF-MS analysis and enzyme affinity toward methoxy and hydroxy substituents on the benzene ring revealed that the AtFAE-1 belonged to type A while AtFAE-2 and AtFAE-3 were type C FAE."
According to the news editors, the research concluded: "The FAEs could release 65 to 90% of ferulic acid from agrowaste substrates in the presence of xylanase."
For more information on this research see: Purification and biochemical characterization of feruloyl esterases from Aspergillus terreus MTCC 11096. Biotechnology Progress, 2013;29(4):924-932. Biotechnology Progress can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (American Chemical Society - www.acs.org; Biotechnology Progress - www.pubs.acs.org/journal/bipret)
The news editors report that additional information may be obtained by contacting C.G. Kumar, Acharya Nagarjuna Univ, Dept. of Biotechnol, Guntur 522510, Andhra Pradesh, India. Additional authors for this research include A. Kamle and A. Kamal (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, Chemistry, Esterases, Hydrolases, Biochemical, Andhra Pradesh, Enzymes and Coenzymes
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