By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biotechnology have been published. According to news reporting out of Harbin, People's Republic of China, by NewsRx editors, research stated, "The present study was undertaken to evaluate the ability of DNA vaccination to protect chickens against reticuloendotheliosis virus (REV) infection and to determine whether codon optimization and the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) could improve the immunogenicity of the DNA vaccines. The wild-type and codon-optimized gp90 genes of REV were cloned into pCAGGS vector, and designated as pCAGgp90 and pCAGoptigp90, respectively."
Our news journalists obtained a quote from the research from Harbin Veterinary Research Institute, "Plasmids pCAGWgp90 and pCAGWoptigp90 containing WPRE were also constructed. To evaluate vaccine efficacy, 3-week-old specific pathogen free chickens were injected with the constructed plasmids twice at 3-week intervals and challenged with REV 3 weeks post boost. Plasmids pCAGoptigp90 and pCAGWgp90 elicited significantly higher humoral and cellular immune responses than pCAGgp90, while chickens immunized with pCAGWoptigp90 showed the highest immune responses among the groups. Chickens immunized with pCAGgp90, pCAGoptigp90, pCAGWgp90 or pCAGWoptigp90 had 53%, 67%, 73% or 87% protection, respectively, as evidenced by the absence of REV viremia, while the empty vector pCAGGS only conferred 13% protection against viremia."
According to the news editors, the research concluded: "These results highlight the potential value of DNA vaccination in the prevention of REV infection and suggest that codon optimization and WPRE could increase the efficacy of DNA vaccines."
For more information on this research see: Protection of chickens against reticuloendotheliosis virus infection by DNA vaccination. Veterinary Microbiology, 2013;166(1-2):59-67. Veterinary Microbiology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Veterinary Microbiology - www.elsevier.com/wps/product/cws_home/503320)
Our news journalists report that additional information may be obtained by contacting K. Li, Chinese Academy Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Avian Infect Dis, Harbin 150001, People's Republic of China. Additional authors for this research include L. Gao, H.L. Gao, X.L. Qi, Y.L. Gao, L.T. Qin, Y.Q. Wang and X.M. Wang (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Harbin, Virology, Viral DNA, rev Genes, RNA Viruses, Viral Genes, DNA Research, DNA Vaccines, Retroviridae, Gammaretrovirus, Genetic Phenomena, Genome Components, Oncogenic Viruses, Genetic Structures, Synthetic Vaccines, Vertebrate Viruses, People's Republic of China
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