By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Enzymes and Coenzymes is the subject of a report. According to news reporting originating in Tamil Nadu, India, by NewsRx journalists, research stated, "Transgenic rice (Oryza sativa L. subsp. indica cv. White Ponni) constitutively expressing the rice thaumatin-like protein gene (tlp-D34, PR-5) individually or in combination with the rice chitinase gene (chi11, PR-3) was generated using an Agrobacterium vir helper strain with multiple copies of pTiBo542 virB and virG."
The news reporters obtained a quote from the research from Madurai Kamaraj University, "Transformation with the tlp-D34 gene alone and tlp-D34 + chi11 genes yielded five and seven single-copy transgenic lines, respectively. Southern blot analysis with two probes, one flanking the right T-DNA border and the second flanking the left T-DNA border, confirmed that all transgenic plants harboured single and complete T-DNA copies. Homozygous transgenic lines were first identified in the T-1 generation by Southern blot analysis and were subsequently confirmed by segregation analysis of T-2 plants. Accumulation of transcripts encoded by the transgenes was confirmed in T-0 plants and homozygous T-2 plants by Northern blot analysis. The homozygous T-2 plants harbouring tlp-D34 + chi11 genes showed 2.8- to 4.2-fold higher chitinase activity. Western blot analysis revealed the accumulation of thaumatin-like protein and chitinase in the respective transgenic plants. Upon infection with Rhizoctonia solani, the disease index reduced from 100 % in control plants to 65 % in a T-3 homozygous transgenic line T4 expressing the tlp-D34 gene alone."
According to the news reporters, the research concluded: "In a T-2 homozygous transgenic line CT22 co-expressing tlp-D34 and chi11 genes, the disease index reduced to 39 %."
For more information on this research see: Transgenic rice lines constitutively co-expressing tlp-D34 and chi11 display enhancement of sheath blight resistance. Biologia Plantarum, 2013;57(2):351-358. Biologia Plantarum can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Biologia Plantarum - www.springerlink.com/content/0006-3134/)
Our news correspondents report that additional information may be obtained by contacting J.M. Shah, Madurai Kamaraj Univ, Sch Biotechnol, Dept. of Plant Biotechnol, Madurai 625021, Tamil Nadu, India. Additional authors for this research include R. Singh and K. Veluthambi (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, Chitinase, Tamil Nadu, Glycoside Hydrolases, Enzymes and Coenzymes
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