By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Life Science Research have been presented. According to news reporting out of Shaanxi, People's Republic of China, by NewsRx editors, research stated, "Proper preservation of transgenes and transgenic materials is important for wider use of transgenic technology in plants. Here, we report stable preservation and faithful expression of a transgene via artificial seed technology in alfalfa."
Our news journalists obtained a quote from the research from Northwest A&F University, "DNA constructs containing the uid reporter gene coding for ?-glucuronidase (GUS) driven by a 35S promoter or a tCUP promoter were introduced into alfalfa via Agrobacterium-mediated genetic transformation. Somatic embryos were subsequently induced from transgenic alfalfa plants via in vitro technology. These embryos were treated with abscisic acid to induce desiccation tolerance and were subjected to a water loss process. After the desiccation procedure, the water content in dried embryos, or called artificial seeds, was about 12-15% which was equivalent to that in true seeds. Upon water rehydration, the dried somatic embryos showed high degrees of viability and exhibited normal germination. Full plants were subsequently developed and recovered in a greenhouse. The progeny plants developed from artificial seeds showed GUS enzyme activity and the GUS expression level was comparable to that of plants developed from somatic embryos without the desiccation process. Polymerase chain reaction analysis indicated that the transgene was well retained in the plants and Southern blot analysis showed that the transgene was stably integrated in plant genome. The research showed that the transgene and the new trait can be well preserved in artificial seeds and the progeny developed."
According to the news editors, the research concluded: "The research provides a new method for transgenic germplasm preservation in different plant species."
For more information on this research see: Preservation and faithful expression of transgene via artificial seeds in alfalfa. Plos One, 2013;8(5):e56699. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting W. Liu, College of Life Sciences, Northwest A & F University, Shaanxi, People's Taiwan. Additional authors for this research include Z. Liang, X. Wang, S. Sibbald, D. Hunter and L. Tian (see also Life Science Research).
Keywords for this news article include: Asia, Shaanxi, Technology, Life Science Research, People's Republic of China.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC