By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Biotechnology is the subject of a report. According to news reporting out of Hubei, People's Republic of China, by NewsRx editors, research stated, "Toxoplasma gondii is one of the most prevalent intracellular parasites and is threatening the health of both humans and animals, therefore causing incalculable economic losses worldwide. Vaccination is thought to be an efficient way of controlling toxoplasmosis. T. gondii microneme protein 11 (MIC11) is a soluble microneme protein which is presumably considered facilitating the early stage of cell invasion."
Our news journalists obtained a quote from the research from Huazhong Agricultural University, "To evaluate the protective efficacy of T. gondii MIC11, in the present study, a new DNA vaccine-encoding the alpha-chain of T. gondii MIC11 was constructed using the pcDNA3.1 vector. Expression of MIC11 from this vector was confirmed by indirect immunofluorescence assay following transfection into baby hamster kidney (BHK) cells. Intramuscular immunization of BALB/c mice with pcDNA/MIC11 was carried out to evaluate the immune responses by serum antibodies titers, lymphoproliferation assay, and cytokines assay. The protective efficacy was evaluated by survival rate in mice after challenging with highly virulent strain of T. gondii. The results demonstrated that this vaccination elicited significant humoral responses and T. gondii lysate antigen (TLA)-stimulated lymphoproliferation (p < 0.05). Compared to controls, the pcDNA/MIC11 immunized mice had high production of IFN-gamma, IL-12, and IL-2 (p < 0.05), but not IL-4 (p > 0.05), indicating that a predominant Th1 type response was developed. The vaccination also increased the survival rate of immunized mice when they were challenged with a lethal dose of tachyzoites of T. gondii RH strain."
According to the news editors, the research concluded: "These data suggest that T. gondii MIC11 is a reasonable vaccine candidate deserving further studies, and pcDNA/MIC11 is a potential strategy for the control of toxoplasmosis."
For more information on this research see: Protective immunity induced by a DNA vaccine-encoding Toxoplasma gondii microneme protein 11 against acute toxoplasmosis in BALB/c mice. Parasitology Research, 2013;112(8):2871-2877. Parasitology Research can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Parasitology Research - www.springerlink.com/content/0932-0113/)
Our news journalists report that additional information may be obtained by contacting Q. Tao, Huazhong Agricultural University, State Key Lab Agr Microbiol, Coll Vet Med, Wuhan 430070, Hubei, People's Republic of China. Additional authors for this research include R. Fang, W.C. Zhang, Y.F. Wang, J.X. Cheng, Y.L. Li, K. Fang, M.K. Khan, M. Hu, Y.Q. Zhou and J.L. Zhao (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Hubei, DNA Research, DNA Vaccines, Synthetic Vaccines, People's Republic of China
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