By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Investigators discuss new findings in Bacterial Infections. According to news reporting from Bethesda, Maryland, by NewsRx journalists, research stated, "Currently available infectious disease imaging techniques cannot differentiate between infection and sterile inflammation or between different types of infections. Recently, radiolabeled FIAU was found to be a substrate for the thymidine kinase (TK) enzyme of multiple pathogenic bacteria, leading to its translational use in the imaging of bacterial infections."
The news correspondents obtained a quote from the research from the National Institutes of Health, "Patients with immunodeficiencies, however, are susceptible to a different group of pathogenic bacteria when compared to immunocompetent subjects. In this study, we wanted to predict the usefulness of radiolabeled FIAU in the detection of bacterial infections commonly occurring in patients with immunodeficiencies, in vitro, prior to attempting in vivo imaging with (124)I-FIAU-PET. We obtained representative strains of bacterial pathogens isolated from actual patients with genetic immunodeficiencies. We evaluated the bacterial susceptibility of different strains to the effect of incubation with FIAU, which would implicate the presence of the thymidine kinase (TK) enzyme. We also incubated the bacteria with (14)C-FIAU and consequently measured its rate of incorporation in the bacterial DNA using a liquid scintillation counter. Unlike the other bacterial strains, the growth of Pseudomonas aeruginosa was not halted by FIAU at any concentration. All the tested clinical isolates demonstrated different levels of (14)C-FIAU uptake, except for P. aeruginosa. Radiolabeled FIAU has been successful in delineating bacterial infections, both in preclinical and pilot translational studies. In patients with immunodeficiencies, Pseudomonas infections are commonly encountered and are usually difficult to differentiate from fungal infections. The use of radiolabeled FIAU for in vivo imaging of those patients, however, would not be useful, considering the apparent lack of TK enzyme in Pseudomonas. One has to keep in mind that not all pathogenic bacteria possess the TK enzyme and as such will not all retain FIAU."
According to the news reporters, the research concluded: "Our technique is simple, and can be easily used to assess whether a certain bacterial strain of interest can or cannot be visualized using radiolabeled FIAU."
For more information on this research see: The use of 14C-FIAU to predict bacterial thymidine kinase presence: implications for radiolabeled FIAU bacterial imaging. Nuclear Medicine and Biology, 2013;40(5):638-42. (Elsevier - www.elsevier.com; Nuclear Medicine and Biology - www.elsevier.com/wps/product/cws_home/525482)
Our news journalists report that additional information may be obtained by contacting K.L. Peterson, Center for Infectious Disease Imaging, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD 20814-9692, United States. Additional authors for this research include W.C. Reid, A.F. Freeman, S.M. Holland, R.I. Pettigrew, A.M. Gharib and D.A Hammoud (see also Bacterial Infections).
Keywords for this news article include: Bethesda, Maryland, Genetics, Proteins, Proteomics, United States, Pseudomonadaceae, Thymidine Kinase, Gammaproteobacteria, Enzymes and Coenzymes, Pseudomonas Infections, North and Central America, Bacterial Infections and Mycoses, Gram Negative Bacterial Infections, Gram Negative Aerobic Rods and Cocci.
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