By a News Reporter-Staff News Editor at Journal of Technology -- Current study results on Epithelial Cells have been published. According to news reporting originating from Ljubljana, Slovenia, by VerticalNews correspondents, research stated, "Appropriate assessment of transepithelial permeability in vitro is needed to estimate and model transmucosal bioavailability to achieve oral delivery of protein biopharmaceuticals. The Caco-2 cell-based intestinal epithelium model is widely used for this purpose for low molecular mass drugs."
Our news editors obtained a quote from the research from the University of Ljubljana, "The aim of this study was to test the suitability of the Caco-2 model for assessing enhanced transepithelial permeability to proteins. Four unrelated proteins were chosen to test the permeability of Caco-2 monolayers. It was found that proteins could cross the epithelium model, in spite of their size. All tested proteins had similar very low apparent permeability coefficients (P-app) of around 4 x 10(-10) cm/s. Protein stability over three-hour exposure to Caco-2 was also confirmed. Their crossing rate in a cell-free setup was also measured, to determine the upper limit of permeability to proteins. An epithelium permeability enhancer N-decyl-beta-D-maltopyranoside (MP C10) was used to demonstrate accelerated permeability conditions. Papp values could be increased dose dependently up to about 1 x 10(-7) cm/s, close to the level in the cell-free setup, indicating distinctive potential of the model. This along with enhancing effect of known specific route permeators suggests involvement of the paracellular route in protein transport."
According to the news editors, the research concluded: "Our results thus indicate that the Caco-2 model is a suitable tool for in vitro assessment of enhanced permeability to proteins."
For more information on this research see: The Caco-2 cell culture model enables sensitive detection of enhanced protein permeability in the presence of N-decyl-beta-D-maltopyranoside. New Biotechnology, 2013;30(5):507-515. New Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; New Biotechnology - www.elsevier.com/wps/product/cws_home/713354)
The news editors report that additional information may be obtained by contacting M. Marusic, Univ Ljubljana, Biotech Fac, Dept. of Biol, Ljubljana, Slovenia. Additional authors for this research include T. Zupancic, G. Hribar, R. Komel, G. Anderluh and S. Caserman.
Keywords for this news article include: Europe, Slovenia, Peptides, Proteins, Ljubljana, Amino Acids, Caco-2 Cells, Epithelial Cells
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