By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators publish new report on Biotechnology. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "Gastric cancer stem cells (CSCs), which require activation of Wnt signaling to maintain their self-renewal and tumorigenicity, are proposed to be critical targets for effective therapy of gastric carcinomas. Gene therapies that are delivered by adenovirus of serotype 5 (Ad5) or chimeric 5/35(Ad5/35) adenovirus have shown promise for treating various cancers."
Our news journalists obtained a quote from the research from Chongqing University, "Here we aimed to develop a gene therapy strategy that targeted gastric CSCs (CD44(+) cells). CD44(+) cells were isolated by fluorescence activated cell sorting from both primary gastric cancer cells and cell lines. Expression of adenovirus receptors was examined in CD44(+) and CD44(-) cells. A potent Wnt antagonist Dickkopf-1 (DKK1) was delivered into CD44(+) cells using Ad5/35 (Ad5/35-DKK1). The therapeutic outcomes were evaluated. Expression of Coxsakievirus adenovirus receptor for Ad5 was significantly reduced, while abundance of CD46, the receptor for Ad5/35, was slightly higher in CD44(+) cells. Accordingly, CD44(+) cells were sensitive to Ad5/35 infection, but not to Ad5. Ad5/35-DKK1 introduced DKK1 into CD44(+) cells and deactivated endogenous Wnt/beta-catenin signaling efficiently. Overexpression of DKK1 inhibited survival, anchorage-independent colony formation, and invasion of CD44(+) cells, which were restored by a GSK-3 specific inhibitor BIO-acetoxime. More importantly, introduction of DKK1 abrogated the tumorigenicity of CD44(+) cells in vivo. However, Ad5/35-DKK1 only showed minimal cytotoxicity to normal tissue-derived cells, L-02 and GES-1."
According to the news editors, the research concluded: "We developed, for the first time, a novel Ad5/35-DKK1-based approach to abrogate Wnt signaling in CSCs and demonstrated that gastric CSC-targeting gene therapy was effective in preclinical experiments."
For more information on this research see: Chimeric 5/35 adenovirus-mediated Dickkopf-1 overexpression suppressed tumorigenicity of CD44(+) gastric cancer cells via attenuating Wnt signaling. Journal of Gastroenterology, 2013;48(7):798-808. Journal of Gastroenterology can be contacted at: Springer Japan Kk, Chiyoda First Bldg East, 3-8-1 Nishi-Kanda, Chiyoda-Ku, Tokyo, 101-0065, Japan. (Springer - www.springer.com; Journal of Gastroenterology - www.springerlink.com/content/0944-1174/)
Our news journalists report that additional information may be obtained by contacting B. Wang, Chongqing Univ, Bioengn College, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, People's Republic of China. Additional authors for this research include J. Liu, L.N. Ma, H.L. Xiao, Y.Z. Wang, Y. Li, Z. Wang, L.L. Fan, C.H. Lan, M. Yang, L. Hu, Y.L. Wei, X.W. Bian, D.F. Chen and J. Wang (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Oncology, Chongqing, Adenovirus, Bioengineering, Gastric Cancer, Gastroenterology, Cancer Gene Therapy, People's Republic of China
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