By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Biological Factors are discussed in a new report. According to news reporting out of Oberschleissheim, Germany, by NewsRx editors, research stated, "An automated flow-through multi-mycotoxin immunoassay using the stand-alone Munich Chip Reader 3 platform and reusable biochips was developed and evaluated. This technology combines a unique microarray, prepared by covalent immobilization of target analytes or derivatives on diamino-poly(ethylene glycol) functionalized glass slides, with a dedicated chemiluminescence readout by a CCD camera."
Our news journalists obtained a quote from the research from the University of Munich, "In a first stage, we aimed for the parallel detection of aflatoxins, ochratoxin A, deoxynivalenol, and fumonisins in cereal samples in a competitive indirect immunoassay format. The method combines sample extraction with methanol/water (80:20, v/v), extract filtration and dilution, and immunodetection using horseradish peroxidase-labeled anti-mouse IgG antibodies. The total analysis time, including extraction, extract dilution, measurement, and surface regeneration, was 19 min. The prepared microarray chip was reusable for at least 50 times. Oat extract revealed itself as a representative sample matrix for preparation of mycotoxin standards and determination of different types of cereals such as oat, wheat, rye, and maize polenta at relevant concentrations according to the European Commission regulation. The recovery rates of fortified samples in different matrices, with 55-80 and 58-79 %, were lower for the better water-soluble fumonisin B1 and deoxynivalenol and with 127-132 and 82-120 % higher for the more unpolar aflatoxins and ochratoxin A, respectively."
According to the news editors, the research concluded: "Finally, the results of wheat samples which were naturally contaminated with deoxynivalenol were critically compared in an interlaboratory comparison with data obtained from microtiter plate ELISA, aokinmycontrolA ® method, and liquid chromatography-mass spectrometry and found to be in good agreement."
For more information on this research see: Automated regenerable microarray-based immunoassay for rapid parallel quantification of mycotoxins in cereals. Analytical and Bioanalytical Chemistry, 2013;405(20):6405-6415. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
Our news journalists report that additional information may be obtained by contacting S. Oswald, University of Munich, Hyg & Technol Milk, D-85764 Oberschleissheim, Germany. Additional authors for this research include X. Karsunke, R. Dietrich, E. Martlbauer, R. Niessner and D. Knopp (see also Biological Factors).
Keywords for this news article include: Europe, Germany, Mycotoxins, Oberschleissheim, Biological Toxins, Biological Factors
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