By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on Stem Cell Research have been published. According to news reporting out of Tehran, Iran, by NewsRx editors, research stated, "Nerve growth factor (NGF), a member of the neurotrophic factor family, plays a critical role in the maintenance and regeneration of different types of neurons. To overcome drastic challenges in the peripheral delivery of NGF, transplantation of NGF secreting stem cells to the target site of an injury may be an effective procedure."
Our news journalists obtained a quote from the research from Tarbiat Modares University, "Unrestricted somatic stem cells (USSCs), a subtype of umbilical cord blood (UCB) stem cells, have shown promise for gene therapy purposes, and proper results have been observed from transplantation experiments in neurodegenerative disorders. Based on the considerable potential of USSCs for gene delivery applications, the goal of the current study was to establish a betaNGF gene containing USSCs, which is able to secrete functional recombinant betaNGF protein. Unrestricted somatic stem cells were isolated from UCB and were cultured in a DMEM medium. The betaNGF gene was cloned in the EFalpha-promoting lentiviral vector, and virus production was performed as a third generation lentivirus packaging system. Enzyme-linked immunosorbent assay, real-time PCR, co-culture, MTT, and immunocytochemistry assays were performed to evaluate the genetically engineered USSCs. Overexpression of betaNGF gene in human USSCs created a USSC line that is able to secrete high amounts of functional betaNGF protein. betaNGF-secreting USSCs showed a high rate of viability along with acceptable immunological and morphological properties for transplantation into the nervous system."
According to the news editors, the research concluded: "Long-term expression of functional betaNGF, high viability of betaNGF producing USSCs, and expression of primary neuronal markers suggest that USSCs may be useful to deliver betaNGF into targeted sites of the nervous system in neurodegenerative disorders."
For more information on this research see: Unrestricted somatic stem cells as vehicle for nerve growth factor gene transfer. Neurological Research, 2013;35(6):553-560. Neurological Research can be contacted at: Maney Publishing, Ste 1C, Josephs Well, Hanover Walk, Leeds LS3 1AB, W Yorks, England (see also Stem Cell Research).
Our news journalists report that additional information may be obtained by contacting H. Jalali, Tarbiat Modares Univ, Fac Med Sci, Dept. of Hematol, Tehran, Iran. Additional authors for this research include K. Parivar, M. Nabiuni and M. Soleimani.
Keywords for this news article include: Iran, Asia, Biotechnology, Tehran, Gene Therapy, Bioengineering, Stem Cell Research, Nerve Growth Factors, Nerve Tissue Proteins, Intercellular Signaling Peptides and Proteins
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