By a News Reporter-Staff News Editor at Angiogenesis Weekly -- Fresh data on Ischemia are presented in a new report. According to news reporting originating from Seoul, South Korea, by NewsRx correspondents, research stated, "Matrix metalloproteinase (MMP)-2 and MMP-9 have been known to play the role of essential mediators in angiogenesis. Non-invasive in vivo imaging approach using imaging probes is a potential method of detecting MMP activity in living animals, wherein imaging probes must include the characteristics of non-toxicity, specific targetability, and reasonable signal intensity."
Our news editors obtained a quote from the research from Korea University, "Here, we developed MMP-specific and self-quenched human serum albumin (HSA)-based (MMP-HSA) nanoprobes for non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. MMP-specific fluorogenic peptide probes, which were self-quenched with a near-infrared fluorophore and a quencher, were covalently conjugated to HSA (MMP-HSA nanoprobes). MMP-HSA nanoprobes formed stable nanoparticle structures of approximately 36 nm in diameter. Strongly self-quenched MMP-HSA nanoprobes boosted intense fluorescence signals in the presence of MMP-2 and MMP-9. Furthermore, MMP-HSA nanoprobes showed no cytotoxicity in cell culture. Importantly, intravenous injection of MMP HSA nanoprobes provided longer blood half-life and successful non-invasive optical imaging of MMP activity during angiogenesis in the mouse hindlimb ischemia model. In addition, the MMP activity visualized by MMP-HSA nanoprobes was consistent with the results of zymography, Western blot, and immunohistochemistry."
According to the news editors, the research concluded: "MMP-HSA nanoprobes may be useful for monitoring of the initial process of angiogenesis through non-invasive MMP imaging."
For more information on this research see: Non-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model. Biomaterials, 2013;34(28):6871-6881. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news editors report that additional information may be obtained by contacting J.H. Ryu, Korea University, KU KIST Sch, Seoul 136701, South Korea. Additional authors for this research include J.Y. Shin, S.A. Kim, S.W. Kang, H. Kim, S. Kang, K. Choi, I.C. Kwon, B.S. Kim and K. Kim (see also Ischemia).
Keywords for this news article include: Asia, Seoul, Ischemia, Proteomics, South Korea, Angiogenesis, Peptide Hydrolases, Enzymes and Coenzymes, Metalloendopeptidases, Matrix Metalloproteinases
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