By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Microbiology is the subject of a report. According to news reporting from Gauhati, India, by NewsRx journalists, research stated, "An extracellular cutinase from Pseudomonas cepacia NRRL B 2320 was purified to apparent homogeneity. Upon biochemical characterization, the purified cutinase was found to be tolerant to organic solvents and surfactants under assay conditions."
The news correspondents obtained a quote from the research from the Indian Institute of Technology, "The molecular mass of cutinase was found to be 26.25 kDa by MALDI-TOF-MS analysis. The enzyme was able to show activity towards synthetic esters of chain length C-4-C-16. The activity of cutinase was enhanced by mono cations and various effectors, whereas it was moderately inhibited by various divalent cations and serine blocking reagent, phenyl methyl sulphonyl fluoride (PMSF). The optimal pH and temperature for highest activity were found to be 7.9 and 36.5 degrees C, respectively. An overall 1.42-fold increase in activity was observed after optimization of both assay and process conditions. The exposure of hydrophobic amino acid to an aqueous environment and change in secondary structure of cutinase was observed from thermodynamic parameters (Delta H*, Delta S*), fluorescence and circular dichorism spectra during the deactivation process."
According to the news reporters, the research concluded: "Two cutinase encoding genes were identified in P. cepacia, cloned and expressed in E. coli BL21 (DE3)."
For more information on this research see: Novel cutinase from Pseudomonas cepacia NRRL B 2320: Purification, characterization and identification of cutinase encoding genes. Journal of General and Applied Microbiology, 2013;59(3):171-184. Journal of General and Applied Microbiology can be contacted at: Microbiol Res Foundation, Japan Academic Soc Center Bldg 4-16 Yayoi 2-Chome, Tokyo, Tokyo 113, Japan (see also Microbiology).
Our news journalists report that additional information may be obtained by contacting K. Dutta, Indian Inst Technol IIT Guwahati, Dept. of Biotechnol, Biochem Engn Lab, Gauhati 781039, Assam, India. Additional authors for this research include H. Krishnamoorthy and V.V. Dasu.
Keywords for this news article include: Asia, India, Gauhati, Microbiology
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