technology Target Subtilisins -->
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting originating from New Delhi, India, by NewsRx correspondents, research stated, "Egress of Plasmodium falciparum merozoites from host erythrocytes is a critical step in multiplication of blood-stage parasites. A cascade of proteolytic events plays a major role in degradation of membranes leading to egress of merozoites."
Our news editors obtained a quote from the research from International Center for Genetic Engineering and Biotechnology, "However, the signals that regulate the temporal activation and/or secretion of proteases upon maturation of merozoites in intra-erythrocytic schizonts remain unclear. Here, we have tested the role of intracellular Ca(2+) in regulation of egress of P.?falciparum merozoites from schizonts. A sharp rise in intracellular Ca(2+) just before egress, observed by time-lapse video microscopy, suggested a role for intracellular Ca(2+) in this process. Chelation of intracellular Ca(2+) with chelators such as BAPTA-AM or inhibition of Ca(2+) release from intracellular stores with a phospholipase C (PLC) inhibitor blocks merozoite egress. Interestingly, chelation of intracellular Ca(2+) in schizonts was also found to block the discharge of a key protease PfSUB1 (subtilisin-like protease 1) from exonemes of P.?falciparum merozoites to parasitophorous vacuole (PV). This leads to inhibition of processing of PfSERA5 (serine repeat antigen 5) and a block in parasitophorous vacuolar membrane (PVM) rupture and merozoite egress."
According to the news editors, the research concluded: "A complete understanding of the steps regulating egress of P.?falciparum merozoites may provide novel targets for development of drugs that block egress and limit parasite growth."
For more information on this research see: Ca(2+) -mediated exocytosis of subtilisin-like protease 1: a key step in egress of Plasmodium falciparum merozoites. Cellular Microbiology, 2013;15(6):910-21. (Wiley-Blackwell - www.wiley.com/; Cellular Microbiology - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-5822)
The news editors report that additional information may be obtained by contacting S. Agarwal, Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, New Delhi, India. Additional authors for this research include M.K. Singh, S. Garg, C.E. Chitnis and S. Singh (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, New Delhi, Subtilisins, Serine Proteases, Peptide Hydrolases, Enzymes and Coenzymes, Serine Endopeptidases.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC