By a News Reporter-Staff News Editor at Health & Medicine Week -- Research findings on Mass Spectrometry are discussed in a new report. According to news reporting originating in Beijing, People's Republic of China, by NewsRx journalists, research stated, "In this study, a novel y-type high molecular weight glutenin subunit (HMW-GS) in wild emmer wheat Triticum turgidum L. var. dicoccoides (Korn.) accession KU1952 was identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis (CE) and matrix-assisted laser desorption ionisation/time-of-flight/mass spectrometry (MALDI-TOF-MS). Its electrophoretic mobility and molecular weight were similar to those of 1By16 and was designated as 1By16*."
The news reporters obtained a quote from the research from Capital Normal University, "The complete coding sequence of the 1By16* gene isolated by allelic-specific polymerase chain reaction (AS-PCR) consists of 2,157 bp, encoding 729 amino acid residues. The real presence and authenticity of the 1By16* gene in KU1952 were further confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), heterologous expression and Western blotting. The molecular structure as well as phylogenetic analysis revealed that 1By16* had 21 single-nucleotide polymorphism (SNP) variations and possessed greater similarity with superior quality subunits 1By15 and 1By16 of common wheat. Secondary structure prediction displayed higher ?-helix and ?-strand contents in the 1By16* subunit, which could form a superior gluten structure and, consequently, might have positive effects on dough quality."
According to the news reporters, the research concluded: "Our results suggest that 1By16* is expected to be a new potential gene for wheat quality improvement."
For more information on this research see: Identification and molecular characterisation of HMW glutenin subunit 1By16* in wild emmer. Journal of Applied Genetics, 2012;53(3):249-58. (Springer - www.springer.com; Journal of Applied Genetics - www.springerlink.com/content/1234-1983/)
Our news correspondents report that additional information may be obtained by contacting M. Jin, Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing 100048, People's Taiwan. Additional authors for this research include Z.Z. Xie, P. Ge, J. Li, S.S. Jiang, S. Subburaj, X.H. Li, F.J. Zeller, S.L. Hsam and Y.M Yan (see also Mass Spectrometry).
Keywords for this news article include: Asia, Beijing, Mass Spectrometry, People's Republic of China.
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