By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Enzymes and Coenzymes have been published. According to news reporting out of Beijing, People's Republic of China, by NewsRx editors, research stated, "The determination of peroxidase activities is the basis for enzyme-labeled bioaffinity assays, peroxidase-mimicking DNAzymes- and nanoparticles-based assays, and characterization of the catalytic functions of peroxidase mimetics. Here, a facile, sensitive, and cost-effective solvent polymeric membrane-based peroxidase detection platform is described that utilizes reaction intermediates with different pK(a) values from those of substrates and final products."
Our news journalists obtained a quote from the research from the Chinese Academy of Sciences, "Several key but long-debated intermediates in the peroxidative oxidation of o-phenylenediamine (o-PD) have been identified and their charge states have been estimated. By using a solvent polymeric membrane functionalized by an appropriate substituted tetraphenylborate as a receptor, those cationic intermediates could be transferred into the membrane from the aqueous phase to induce a large cationic potential response. Thus, the potentiometric indication of the o-PD oxidation catalyzed by peroxidase or its mimetics can be fulfilled. Horseradish peroxidase has been detected with a detection limit at least two orders of magnitude lower than those obtained by spectrophotometric techniques and traditional membrane-based methods."
According to the news editors, the research concluded: "As an example of peroxidase mimetics, G-quadruplex DNAzymes were probed by the intermediate-sensitive membrane and a label-free thrombin detection protocol was developed based on the catalytic activity of the thrombin-binding G-quadruplex aptamer."
For more information on this research see: Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics. Chemistry-A European Journal, 2013;19(30):9979-9986. Chemistry-A European Journal can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany (see also Enzymes and Coenzymes).
Our news journalists report that additional information may be obtained by contacting X.W. Wang, Univ Chinese Academy Sci, Beijing 100049, People's Republic of China. Additional authors for this research include W. Qin.
Keywords for this news article include: Asia, Beijing, Peroxidases, Oxidoreductases, Enzymes and Coenzymes, People's Republic of China
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