By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Nanoparticle Research. According to news reporting originating from Montevideo, Uruguay, by NewsRx correspondents, research stated, "The high incidence and severity of diseases which involve smooth muscle dysfunction dictates the need of continued search for novel therapeutic strategies to treat these conditions. Dendrimers are branched macromolecules with multiple end-groups that can be functionalized for applications which include drug delivery."
Our news editors obtained a quote from the research from the University of the Republic, "There is no data regarding the cellular uptake mechanisms used by dendrimers in smooth muscle human myometrial cells (HMC). Polyamidoamine G4 dendrimers were conjugated with fluorescein isothiocyanate (FITC) and the resulting conjugate (G4-FITC) was characterized using high-performance liquid chromatography, nuclear magnetic resonance, and atomic force microscopy. G4-FITC showed to have no significant effect on the primary culture HMC viability up to 48 h. HMC incubated with G4-FITC were analyzed by laser confocal microscopy. Peri-nuclear fluorescence distribution was observed at 5 h of incubation or more (24, 36, and 48 h). At 24 h, G4-FITC partially co-localized with lysotracker. Uptake of G4-FITC by HMC was slightly inhibited by filipin (8.0 +/- 3.9 %) and significantly inhibited by chlorpromazine (63.5 +/- 3.7 %). In non-electroporated HMC, G4-FITC was never observed inside the cell nucleus. Interestingly, we detected G4-FITC inside the nuclear domain of some electroporated cells. Thus, electroporation changed intracellular G4-FITC localization. Isolated nuclei of HMC incubated with G4-FITC showed fluorescence signal inside the nuclear domain."
According to the news editors, the research concluded: "The results suggest that in HMC, G4-FITC is taken up by clathrin-mediated endocytosis with endosomal and lysosomal localization at 24 h. The combination of electroporation and dendrimers could be an interesting technology to electrotransfer drugs into smooth muscle cells cytosol and nuclei."
For more information on this research see: Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells. Journal of Nanoparticle Research, 2013;15(7):257-270. Journal of Nanoparticle Research can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Journal of Nanoparticle Research - www.springerlink.com/content/1388-0764/)
The news editors report that additional information may be obtained by contacting N. Oddone, University of the Republic, Fac Ciencias, Grp Quim Med, Inst Quim BiolFac Quim, Montevideo 11400, Uruguay. Additional authors for this research include A.I. Zambrana, M. Tassano, W. Porcal, P. Cabral and J.C. Benech (see also Nanoparticle Research).
Keywords for this news article include: Uruguay, Montevideo, Therapeutics, South America, Nanoparticle Research
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