By a News Reporter-Staff News Editor at Immunotherapy Weekly -- Investigators discuss new findings in Intercellular Signaling Peptides and Proteins. According to news originating from Baltimore, Maryland, by NewsRx correspondents, research stated, "BackgroundExposure to environmental endocrine-disrupting chemicals (EDCs) is associated with allergy, chronic inflammation, and immunodeficiency. Phthalates, the common EDCs used in plastic industry, may act as adjuvants to disrupt immune system and enhance allergy."
Our news journalists obtained a quote from the research from Johns Hopkins University, "Plasmacytoid DCs (pDCs) are predominant cells secreting type I interferon (IFN) against infection and are professional antigen-presenting cells in regulating adaptive immunity. However, the effects of phthalates on the function of pDCs are unknown. MethodsCirculating pDCs were isolated from healthy subjects, were pretreated with diethylhexyl phthalate (DEHP) and butyl benzyl phthalate (BBP), and were stimulated with Toll-like receptor (TLR)-9 agonist CpG. IFN-/IFN- levels, surface markers, and T-cell stimulatory function were investigated using ELISA, flow cytometry, and pDC/T-cell coculture assay. Mechanisms were investigated using receptor antagonists, pathway inhibitors, Western blotting, and chromatin immunoprecipitation. ResultsDiethylhexyl phthalate and butyl benzyl phthalate suppressed CpG-induced IFN-/IFN- expression in pDCs, and the effect was reversed by aryl hydrocarbon receptor (AHR) antagonist. Diethylhexyl phthalate suppressed CpG-activated mitogen-activated protein kinase (MAPK)-MEK1/2-ERK-ELK1 and NFB signaling pathways. Diethylhexyl phthalate suppressed CpG-induced interferon regulatory factor (IRF)-7 expression by suppressing histone H3K4 trimethylation at IRF7 gene promoter region through inhibiting translocation of H3K4-specific trimethyltransferase WDR5 from cytoplasm into nucleus. Butyl benzyl phthalate or diethylhexyl phthalate-treated pDCs suppressed IFN- but enhanced IL-13 production by CD4+ T cells."
According to the news editors, the research concluded: "ConclusionPhthalates may interfere with immunity against infection and promote the deviation of Th2 response to increase allergy by acting on human pDCs via suppressing IFN-/IFN- expression and modulating the ability to stimulate T-cell responses."
For more information on this research see: Phthalates suppress type I interferon in human plasmacytoid dendritic cells via epigenetic regulation. Allergy, 2013;68(7):870-879. Allergy can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Allergy - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1398-9995)
The news correspondents report that additional information may be obtained from C.H. Kuo, Johns Hopkins University, Sch Med, Johns Hopkins Asthma & Allergy Center, Baltimore, MD, United States. Additional authors for this research include C.C. Hsieh, H.F. Kuo, M.Y. Huang, S.N. Yang, L.C. Chen, S.K. Huang and C.H. Hung (see also Intercellular Signaling Peptides and Proteins).
Keywords for this news article include: Antigen-Presenting Cells, Maryland, Genetics, Baltimore, Cytokines, Immunology, Interferons, United States, Phthalic Acids, Dendritic Cells, Diethylhexyl Phthalate, North and Central America, Mononuclear Phagocyte System, Intercellular Signaling Peptides and Proteins
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