Scientists use tools called gradients to understand how molecules interact in biological systems. Researchers from
The new technique begins with a substrate made of indium gallium nitride. Negatively-charged oxides form on the surface, which then bind to the amino acid L-arginine. Image credit:
A gradient is a material that has a specific molecule on its surface, with the concentration of the molecule sloping from a high concentration on one end to a low concentration at the other end. The gradient is used not only to determine whether other molecules interact with the molecules on the gradient, but to determine the threshold level at which any interactions take place.
The new technique begins by creating a substrate, prepared in the lab of
The researchers then put the substrate into a solution that contains an amino acid called L-arginine, which is positively charged at biologically relevant pH levels -- such as those found in the human body.
"The L-arginine binds to the negatively charged oxides on the surface of the substrate," says
"We studied L-arginine because it is small, but relevant. Because it is small, we could easily assess what was happening during our study," Bain says. "But because it is a building block for proteins, we can build on this work to study full peptides and proteins -- such as ligands that bind to cell receptors."
"This technique also creates changes in the topography of the InGaN's surface, based on the different crystalline structures within the material as its shifts from being indium-rich to being gallium-rich," says Dr.
The paper, " Biomolecular Gradients via Semiconductor Gradients: Characterization of Amino Acid Adsorption to InxGa1-xN Surfaces ," is published online in ACS Applied Materials and Interfaces. The paper's co-authors include Aadhithya Hosalli Mukund of
Note to Editors: The study abstract follows.
"Biomolecular Gradients via Semiconductor Gradients: Characterization of Amino Acid Adsorption to InxGa1-xN Surfaces"
Published : Online
DOI : 10.1021/am4015555
Abstract: The band gap of indium gallium nitride can be tuned by varying the compositional ratio of indium to gallium, spanning the entire visible region and extending into the near-infrared and near-ultraviolet. This tunability allows for device optimization specific to different applications, including as a biosensor or platform for studying biological interactions. However, these rely on chemically-dependent interactions between the device surface and the biostructures of interest. This study presents a material gradient of changing In:Ga composition and the subsequent evaluation of amino acid adsorption to this surface. Arginine is adsorbed to the surface in conditions both above and below the isoelectric point, providing insight to the role of electrostatic interactions in interface formation. These electrostatics are the driving force of the observed adsorption behaviors, with protonated amino acid demonstrating increased adsorption as a function of native surface oxide buildup. We thus present a gradient inorganic substrate featuring varying affinity for amino acid adhesion, which can be applied in generating gradient architectures for biosensors and studying cellular behaviors without application of specialized patterning processes.
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