By a News Reporter-Staff News Editor at Proteomics Weekly -- Investigators discuss new findings in Quantum Dots. According to news originating from Vancouver, Canada, by NewsRx correspondents, research stated, "Paper-based assays are a promising diagnostic format for point-of-care applications, field deployment, and other low-resource settings. To date, the majority of efforts to integrate nanomaterials with paper-based assays have utilized gold nanoparticles."
Our news journalists obtained a quote from the research from the University of British Columbia, "Here, we show that semiconductor quantum dots (QDs), in combination with Forster resonance energy transfer (FRET), are also suitable nanomaterials for developing paper-based assays. Paper fibers were chemically modified with thiol ligands to immobilize CdSeS/ZnS QDs, the QDs were self-assembled with dye-labeled peptides to generate efficient FRET, and steady-state and fluorescence lifetime imaging microscopy (FLIM) were used for characterization. Peptides were selected as substrates for three different proteases and a series of kinetic assays for proteolytic activity was carried out, including multiplexed assays and pro-enzyme activation assays. Quantitative results were obtained within 5-60 min at levels as low as 1-2 nM of protease. These assays were possible using simple optical readout platforms that did not negate the low cost, ease of use, and overall accessibility advantages of paper-based assays. A violet light-emitting diode (LED) excitation source and color imaging with either a digital camera, consumer webcam, or smartphone camera were sufficient for analysis on the basis of a red/green color intensity ratio. At most, a universal serial bus (USB) connection to a computer was required and the instrumentation cost orders of magnitude less than that typically utilized for in vitro bioanalyses with QDs."
According to the news editors, the research concluded: "This work demonstrates that QDs are valuable probes for developing a new generation of paper-based diagnostics."
For more information on this research see: Proteolytic assays on quantum-dot-modified paper substrates using simple optical readout platforms. Analytical Chemistry, 2013;85(18):8817-25. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from E. Petryayeva, Dept. of Chemistry, University of British Columbia , 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada (see also Quantum Dots).
Keywords for this news article include: Canada, Vancouver, Quantum Dots, Nanotechnology, British Columbia, Emerging Technologies, North and Central America.
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