By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Life Science Research are discussed in a new report. According to news reporting originating from Jinju, South Korea, by NewsRx correspondents, research stated, "Improvement of a microorganism's tolerance against organic solvents is required for a microbial factory producing terpenoid based biofuels. The bacterial genes, marA, imp, cls and cti have been found to increase organic solvent tolerance."
Our news editors obtained a quote from the research from Gyeongsang National University, "Thus, the tolerance against the following terpenoids (isopentenol, geraniol, myrcene, and farnesol) was studied with overexpression of marA, imp, cls and cti genes in Escherichia coli. The marA overexpression significantly enhanced the tolerance of E. coli against geraniol, whereas there was no tolerance improvement against the terpenoids by overexpression of cls and cti genes. The imp overexpression even yielded sensitive phenotype to the tested solvents. The colony forming efficiency of the marA overexpressing E. coli was increased by 10(4)-fold in plate overlay of geraniol compared to that of wild type E. coli and a two-fold decrease of intracellular geraniol accumulation was also observed in liquid culture of geraniol. Single knock-out mutations of marA, or one of the following genes (acrA, acrB and tolC) encoding AcrAB-TolC efflux pump made E. coli hypersensitive to geraniol."
According to the news editors, the research concluded: "The geraniol tolerance conferred by marA overexpression was attributed to the AcrAB-TolC efflux pump that is activated by MarA."
For more information on this research see: Enhancement of geraniol resistance of Escherichia coli by MarA overexpression. Journal of Bioscience and Bioengineering, 2013;115(3):253-8. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
The news editors report that additional information may be obtained by contacting A.A. Shah, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 900 Gajwadong, Jinju, South Korea. Additional authors for this research include C. Wang, Y.R. Chung, J.Y. Kim, E.S. Choi and S.W Kim (see also Life Science Research).
Keywords for this news article include: Asia, Jinju, South Korea, Life Science Research.
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