By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Silicon Nanowires. According to news reporting from Taipei, Taiwan, by NewsRx journalists, research stated, "Understanding how proteins interact with each other is the basis for studying the biological mechanisms behind various physiological activities. Silicon nanowire field-effect transistors (SiNW-FETs) are sensitive sensors used to detect biomolecular interactions in real-time."
The news correspondents obtained a quote from the research from National Taiwan University, "However, the majority of the applications that use SiNW-FETs are for known interactions between different molecules. To explore the capability of SiNW-FETs as fast screening devices to identify unknown interacting molecules, we applied mass spectrometry (MS) to analyze molecules reversibly bound to the SiNW-FETs. Calmodulin (CaM) is a Ca(2+)-sensing protein that is ubiquitously expressed in cells and its interaction with target molecules is Ca(2+)-dependent. By modifying the SiNW-FET surface with glutathione, glutathione S-transferase (GST)-tagged CaM binds reversibly to the SiNW-FET. We first verified the Ca(2+)-dependent interaction between GST-CaM and purified troponin I, which is involved in muscle contraction, through the conductance changes of the SiNW-FET. Furthermore, the cell lysate containing overexpressed Ca(2+)/CaM-dependent protein kinase II? induced a conductance change in the GST-CaM-modified SiNW-FET. The bound proteins were eluted and subsequently identified by MS as CaM and kinase. In another example, candidate proteins from neuronal cell lysates interacting with calneuron I (CalnI), a CaM-like protein, were captured with a GST-CalnI-modified SiNW-FET. The proteins that interacted with CalnI were eluted and verified by MS. The Ca(2+)-dependent interaction between GST-CalnI and one of the candidates, heat shock protein 70, was re-confirmed via the SiNW-FET measurement."
According to the news reporters, the research concluded: "Our results demonstrate the effectiveness of combining MS with SiNW-FETs to quickly screen interacting molecules from cell lysates."
For more information on this research see: Improved silicon nanowire field-effect transistors for fast protein-protein interaction screening. Lab On a Chip - Miniaturisation for Chemistry and Biology, 2013;13(4):676-84 (see also Silicon Nanowires).
Our news journalists report that additional information may be obtained by contacting T.Y. Lin, Institute of Zoology, National Taiwan University, Taipei, Taiwan. Additional authors for this research include B.R. Li, S.T. Tsai, C.W. Chen, C.H. Chen, Y.T. Chen and C.Y Pan.
Keywords for this news article include: Asia, Taipei, Taiwan, Peptides, Proteins, Amino Acids, Nanotechnology, Silicon Nanowires, Emerging Technologies.
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