By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Capillary Electrophoresis. According to news originating from Prague, Czech Republic, by NewsRx correspondents, research stated, "The complexation of buffer constituents with the complexation agent present in the solution can very significantly influence the buffer properties, such as pH, ionic strength, or conductivity. These parameters are often crucial for selection of the separation conditions in capillary electrophoresis or high-pressure liquid chromatography (HPLC) and can significantly affect results of separation, particularly for capillary electrophoresis as shown in Part II of this paper series (Bene, M.; Riesova, M.; Svobodova, J.; Tesa?ova, E.; Dubsky, P.; Ga, B. Anal. Chem. 2013, DOI: 10.1021/ac401381d)."
Our news journalists obtained a quote from the research from Charles University, "In this paper, the impact of complexation of buffer constituents with a neutral complexation agent is demonstrated theoretically as well as experimentally for the model buffer system composed of benzoic acid/LiOH or common buffers (e.g., CHES/LiOH, TAPS/LiOH, Tricine/LiOH, MOPS/LiOH, MES/LiOH, and acetic acid/LiOH). Cyclodextrins as common chiral selectors were used as model complexation agents. We were not only able to demonstrate substantial changes of pH but also to predict the general complexation characteristics of selected compounds. Because of the zwitterion character of the common buffer constituents, their charged forms complex stronger with cyclodextrins than the neutral ones do. This was fully proven by NMR measurements. Additionally complexation constants of both forms of selected compounds were determined by NMR and affinity capillary electrophoresis with a very good agreement of obtained values. These data were advantageously used for the theoretical descriptions of variations in pH, depending on the composition and concentration of the buffer."
According to the news editors, the research concluded: "Theoretical predictions were shown to be a useful tool for deriving some general rules and laws for complexing systems."
For more information on this research see: Complexation of buffer constituents with neutral complexation agents: part I. Impact on common buffer properties. Analytical Chemistry, 2013;85(18):8518-25. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from M. Riesova, Charles University in Prague , Faculty of Science, Dept. of Physical and Macromolecular Chemistry, Prague, Czech Republic. Additional authors for this research include J. Svobodova, Z. Toner, M. Bene, E. Tesa?ova and B. Ga (see also Capillary Electrophoresis).
Keywords for this news article include: Prague, Europe, Czech Republic, Capillary Electrophoresis.
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