By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Mass Spectrometry is the subject of a report. According to news originating from Moscow, Russia, by NewsRx correspondents, research stated, "The etectrospray laser desorption/ionization (ELDI) method is actively used for direct sample analysis and ambient mass spectrometry imaging. The optimizing of laser desorption conditions is essential for this technology."
Our news journalists obtained a quote from the research from the Orekhovich Institute of Biomedical Chemistry, "In this work, we propose using a metal target with a black oxide (Fe3O4) coating to increase the signal in ELDI-MS for peptides and small proteins. The experiments were performed on an LTQ-FT mass spectrometer equipped with a home-made ELDI ion source. A cutter blade with black oxide coating was used as a target. A nitrogen laser was used with the following parameters: 337 nm, pulse duration 4ns, repetition rate 10 Hz, fluence similar to 700J m(-2). More than a five times signal increase was observed for a substance P peptide when a coated and a non-coated metal target were compared. No ion signal was observed for proteins if the same fluence and the standard stainless steel target were used. With the assistance of the Fe304 coated metal target and a relatively low laser fluence (
According to the news editors, the research concluded: "A relatively low Laser fluence (
For more information on this research see: Signal enhancement in electrospray laser desorption/ionization mass spectrometry by using a black oxide-coated metal target and a relatively low laser fluence. European Journal of Mass Spectrometry, 2013;19(4):247-252. European Journal of Mass Spectrometry can be contacted at: Im Publications, 6 Charlton Mill, Charlton, Chichester, W Sussex PO18 0HY, England (see also Mass Spectrometry).
The news correspondents report that additional information may be obtained from A. Kononikhin, Russian Academy Med Sci, Orekhovich Inst Biomed Chem, Moscow 119121, Russia. Additional authors for this research include M.Z. Huang, I. Popov, Y. Kostyukevich, E. Kukaev, A. Boldyrev, A. Spasskiy, I. Leypunskiy, J. Shiea and E. Nikolaev.
Keywords for this news article include: Moscow, Russia, Eurasia, Peptides, Proteins, Amino Acids, Mass Spectrometry
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