By a News Reporter-Staff News Editor at Stem Cell Week -- Fresh data on Stem Cell Research are presented in a new report. According to news reporting from Boston, Massachusetts, by NewsRx journalists, research stated, "Biological computing circuits can enhance our ability to control cellular functions and have potential applications in tissue engineering and medical treatments. Transcriptional activator-like effectors (TALEs) represent attractive components of synthetic gene regulatory circuits, as they can be designed de novo to target a given DNA sequence."
The news correspondents obtained a quote from the research from Harvard University, "We here demonstrate that TALEs can perform Boolean logic computation in mammalian cells. Using a split-intein protein-splicing strategy, we show that a functional TALE can be reconstituted from two inactive parts, thus generating two-input AND logic computation. We further demonstrate three-piece intein splicing in mammalian cells and use it to perform three-input AND computation. Using methods for random as well as targeted insertion of these relatively large genetic circuits, we show that TALE-based logic circuits are functional when integrated into the genome of mouse embryonic stem cells. Comparing construct variants in the same genomic context, we modulated the strength of the TALE-responsive promoter to improve the output of these circuits."
According to the news reporters, the research concluded: "Our work establishes split TALEs as a tool for building logic computation with the potential of controlling expression of endogenous genes or transgenes in response to a combination of cellular signals."
For more information on this research see: Two- and three-input TALE-based AND logic computation in embryonic stem cells. Nucleic Acids Research, 2013;41(21):9967-9975. Nucleic Acids Research can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Nucleic Acids Research - nar.oxfordjournals.org)
Our news journalists report that additional information may be obtained by contacting F. Lienert, Harvard University, Wyss Inst Biol Inspired Engn, Boston, MA 02115, United States. Additional authors for this research include J.P. Torella, J.H. Chen, M. Norsworthy, R.R. Richardson and P.A. Silver (see also Stem Cell Research).
Keywords for this news article include: Boston, Massachusetts, United States, Stem Cell Research, Embryonic Stem Cells, North and Central America
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