By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Immunology have been published. According to news originating from Normal, Illinois, by NewsRx correspondents, research stated, "A rapid and simple SERS-based immunoassay has been developed to overcome diffusion-limited binding kinetics that often impedes rapid analysis in conventional heterogeneous immunoassays. This paper describes the development of an antibody-modified membrane as a flow-through capture substrate for a nanoparticle-enabled SERS immunoassay to enhance antibody-antigen binding kinetics."
Our news journalists obtained a quote from the research from Illinois State University, "A thin layer of gold is plated onto polycarbonate track-etched nanoporous membranes via electroless deposition. Capture antibody is immobilized onto the surface of a gold-plated membrane via thiolate coupling chemistry to serve as a capture substrate. A syringe is then used to actively transport the analyte and extrinsic Raman labels to the capture substrate. The fabrication of the gold-plated membrane is thoroughly investigated and established as a viable capture substrate for a SERS-based immunoassay in the absence of sample/SERS label flow. A syringe pump is used to systematically investigate the effect of flow rate on antibody-antigen binding kinetics and demonstrate that active transport to the capture membrane surface expedites antibody-antigen binding. Mouse IgG and goat anti-mouse IgG are selected as a model antigen-antibody system to establish proof of principle. It is demonstrated that the assay for mouse IgG is reduced from 24 h to 10 min and a 10-fold improvement in detection limit is achieved with the flow assay developed herein relative to the passive, i.e., no flow, assay."
According to the news editors, the research concluded: "Moreover, mouse serum is directly analyzed and IgG level is determined using the flow assay."
For more information on this research see: Accelerated surface-enhanced Raman spectroscopy (SERS)-based immunoassay on a gold-plated membrane. Analytical Chemistry, 2013;85(18):8609-17. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from M.A. Penn, Dept. of Chemistry, Illinois State University , Normal, Illinois 61790, United States. Additional authors for this research include D.M. Drake and J.D Driskell (see also Immunology).
Keywords for this news article include: Antibodies, Normal, Illinois, Immunology, United States, Blood Proteins, Immunoglobulins, North and Central America.
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