By a News Reporter-Staff News Editor at Gene Therapy Weekly -- A new study on Biotechnology is now available. According to news originating from Kyoto, Japan, by NewsRx correspondents, research stated, "Alpha-synuclein (SNCA) is an established susceptibility gene for Parkinson's disease (PD), one of the most common human neurodegenerative disorders. Increased SNCA is considered to lead to PD and dementia with Lewy bodies."
Our news journalists obtained a quote from the research from Kyoto Prefectural University, "Four single-nucleotide polymorphisms (SNPs) in SNCA 3' region were prominently associated with PD among different ethnic groups. To examine how these SNPs influence disease susceptibility, we analyzed their potential effects on SNCA gene expression. We found that rs356219 showed allele-specific features. Gel shift assay using nuclear extracts from SH-SY5Y cells showed binding of one or more proteins to the protective allele, rs356219-A. We purified the rs356219-A-protein complex with DNA affinity beads and identified a bound protein using mass spectrometry. This protein, YY1 (Yin Yang 1), is an ubiquitous transcription factor with multiple functions. We next investigated SNCA expression change in SH-SY5Y cells by YY1 transfection. We also analyzed the expression of antisense noncoding RNA (ncRNA) RP11-115D19.1 in SNCA 3'-flanking region, because rs356219 is located in intron of RP11-115D19.1. Little change was observed in SNCA expression levels; however, RP11-115D19.1 expression was prominently stimulated by YY1. In autopsied cortices, positive correlation was observed among RP11-115D19.1, SNCA and YY1 expression levels, suggesting their functional interactions in vivo. Knockdown of RP11-115D19.1 increased SNCA expression significantly in SH-SY5Y cells, suggesting its repressive effect on SNCA expression."
According to the news editors, the research concluded: "Our findings of the protective allele-specific YY1 and antisense ncRNA raised a novel possible mechanism to regulate SNCA expression."
For more information on this research see: YY1 binds to alpha-synuclein 3 '-flanking region SNP and stimulates antisense noncoding RNA expression. Journal of Human Genetics, 2013;58(11):711-719. Journal of Human Genetics can be contacted at: Nature Publishing Group, 75 Varick St, 9TH Flr, New York, NY 10013-1917, USA. (Nature Publishing Group - www.nature.com/; Journal of Human Genetics - www.nature.com/jhg/)
The news correspondents report that additional information may be obtained from I. Mizuta, Kyoto Prefectural Univ Med, North Med Center, Kyoto, Japan. Additional authors for this research include K. Takafuji, Y. Ando, W. Satake, M. Kanagawa, K. Kobayashi, S. Nagamori, T. Shinohara, C. Ito, M. Yamamoto, N. Hattori, M. Murata, Y. Kanai, S. Murayama, M. Nakagawa and T. Toda (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Antisense Technology, Biotechnology, Kyoto, Japan, Bioengineering, alpha-Synuclein, Nerve Tissue Proteins
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