technology Described by Investigators at Swiss Federal Institute of Technology -->
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Nanotechnology. According to news reporting out of Basel, Switzerland, by NewsRx editors, research stated, "Elucidating the mechanisms by which proteins translocate small molecules and ions through transmembrane pores and channels is of great interest in biology, medicine, and nanotechnology. However, the characterization of pore forming proteins in their native state lacks suitable methods that are capable of high-resolution imaging (similar to 1 nm) while simultaneously mapping physical and chemical properties."
Our news journalists obtained a quote from the research from the Swiss Federal Institute of Technology, "Here we report how force distance (FD) curve-based atomic force microscopy (AFM) imaging can be applied to image the native pore forming outer membrane protein F (OmpF) at subnanometer resolution and to quantify the electrostatic field and potential generated by the transmembrane pore. We further observe the electrostatic field and potential of the OmpF pore switching 'on' and 'off' in dependence of the electrolyte concentration. Because electrostatic field and potential select for charged molecules and ions and guide them to the transmembrane pore the insights are of fundamental importance to understand the pore function."
According to the news editors, the research concluded: "These experimental results establish FD-based AFM as a unique tool to image biological systems to subnanometer resolution and to quantify their electrostatic properties."
For more information on this research see: Quantitative Imaging of the Electrostatic Field and Potential Generated by a Transmembrane Protein Pore at Subnanometer Resolution. Nano Letters, 2013;13(11):5585-5593. Nano Letters can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Nano Letters - www.pubs.acs.org/journal/nalefd)
Our news journalists report that additional information may be obtained by contacting M. Pfreundschuh, Swiss Fed Inst Technol, Dept. of Biosyst Sci & Engn, CH-4058 Basel, Switzerland. Additional authors for this research include U. Hensen and D.J. Muller (see also technology.html">Nanotechnology).
Keywords for this news article include: Basel, Europe, Switzerland, Nanotechnology
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC