By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Germ Cells are presented in a new report. According to news reporting originating in Ithaca, New York, by NewsRx journalists, research stated, "Traditional methods of avian transgenesis involve complex manipulations involving either retroviral infection of blastoderms or the ex vivo manipulation of primordial germ cells (PGCs) followed by injection of the cells back into a recipient embryo. Unlike in mammalian systems, avian embryonic PGCs undergo a migration through the vasculature on their path to the gonad where they become the sperm or ova producing cells."
The news reporters obtained a quote from the research from Cornell University, "In a development which simplifies the procedure of creating transgenic chickens we have shown that PGCs are directly transfectable in vivo using commonly available transfection reagents. We used Lipofectamine 2000 complexed with Tol2 transposon and transposase plasmids to stably transform PGCs in vivo generating transgenic offspring that express a reporter gene carried in the transposon. The process has been shown to be highly effective and as robust as the other methods used to create germ-line transgenic chickens while substantially reducing time, infrastructure and reagents required."
According to the news reporters, the research concluded: "The method described here defines a simple direct approach for transgenic chicken production, allowing researchers without extensive PGC culturing facilities or skills with retroviruses to produce transgenic chickens for wide-ranging applications in research, biotechnology and agriculture."
For more information on this research see: A new method for producing transgenic birds via direct in vivo transfection of primordial germ cells. Transgenic Research, 2013;22(6):1257-1264. Transgenic Research can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Transgenic Research - www.springerlink.com/content/0962-8819/)
Our news correspondents report that additional information may be obtained by contacting S.G. Tyack, Cornell University, Dept. of Microbiol & Immunol, Coll Vet Med, Ithaca, NY 14853, United States. Additional authors for this research include K.A. Jenkins, T.E. O'Neil, T.G. Wise, K.R. Morris, M.P. Bruce, S. McLeod, A.J. Wade, J. McKay, R.J. Moore, K.A. Schat, J.W. Lowenthal and T.J. Doran (see also Germ Cells).
Keywords for this news article include: Ithaca, New York, Genitalia, Germ Cells, United States, North and Central America
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