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By a News Reporter-Staff News Editor at Gene Therapy Weekly -- New research on Capsid is the subject of a report. According to news reporting from Gainesville, Florida, by NewsRx journalists, research stated, "Icosahedral viral capsids are obligated to perform a thermodynamic balancing act. Capsids must be stable enough to protect the genome until a suitable host cell is encountered yet be poised to bind receptor, initiate cell entry, navigate the cellular milieu, and release their genome in the appropriate replication compartment."
The news correspondents obtained a quote from the research from the University of Florida, "In this study, serotypes of adeno-associated virus (AAV), AAV1, AAV2, AAV5, and AAV8, were compared with respect to the physical properties of their capsids that influence thermodynamic stability. Thermal stability measurements using differential scanning fluorimetry, differential scanning calorimetry, and electron microscopy showed that capsid melting temperatures differed by more than 20 degrees C between the least and most stable serotypes, AAV2 and AAV5, respectively. Limited proteolysis and peptide mass mapping of intact particles were used to investigate capsid protein dynamics. Active hot spots mapped to the region surrounding the 3-fold axis of symmetry for all serotypes. Cleavages also mapped to the unique region of VP1 which contains a phospholipase domain, indicating transient exposure on the surface of the capsid. Data on the biophysical properties of the different AAV serotypes are important for understanding cellular trafficking and is critical to their production, storage, and use for gene therapy."
According to the news reporters, the research concluded: "The distinct differences reported here provide direction for future studies on entry and vector production."
For more information on this research see: Comparative Analysis of Adeno-Associated Virus Capsid Stability and Dynamics. Journal of Virology, 2013;87(24):13150-13160. Journal of Virology can be contacted at: Amer Soc Microbiology, 1752 N St NW, Washington, DC 20036-2904, USA. (American Society for Microbiology - www.asm.org; Journal of Virology - jvi.asm.org)
Our news journalists report that additional information may be obtained by contacting V. Rayaprolu, University of Florida, Struct Biol Center, McKnight Brain Inst, Gainesville, FL, United States. Additional authors for this research include S. Kruse, R. Kant, B. Venkatakrishnan, N. Movahed, D. Brooke, B. Lins, A. Bennett, T. Potter, R. McKenna, M. Agbandje-McKenna and B. Bothner (see also Capsid).
Keywords for this news article include: Biotechnology, Virion, Florida, Viruses, Virology, Gainesville, Gene Therapy, Nucleocapsid, United States, Bioengineering, Adeno-Associated Virus, North and Central America
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