By a News Reporter-Staff News Editor at Cancer Weekly -- Investigators publish new report on Myeloid Cells. According to news reporting out of Kharagpur, India, by NewsRx editors, research stated, "The purpose of this study is to evaluate the prospect of using surface modified cobalt oxide(CoO) nanoparticles as carriers of cancerantigens to human macrophages. N-Phosnomethyliminodiacetic acid (PMIDA) was used for surface modification to overcome the toxic effect of CoO nanoparticles."
Our news journalists obtained a quote from the research from the Indian Institute of Technology, "Here, the phosphonate group of the PMIDA acts as a surface-anchoring agent and the remaining -COOH groups bind nonspecifically with tumor associated antigens. This modification allows the conjugation of human oral carcinoma (KB) cell lysate (CL) as an antigen with PMIDA coated CoO nanoparticles (CL-PMIDA-CoO). Particle characterization was performed by dynamic light scattering, atomic force microscopy, and scanning electron microscopy studies. Fourier transform IR spectroscopy was used to investigate conjugation of the protein with nanoparticles. Protein encapsulation was confirmed by protein gel electrophoresis. Active uptake of antigen-conjugated nanoparticles by macrophages was confirmed by fluorescence microscopy. The antitumor activity of the nanocomplex pulsed macrophages was investigated on a human oral carcinoma cell line (KB) in vitro. The modified nanocomplexes upregulate IFN-gamma and TNF-alpha and induce an anticancer immune response by activating macrophages. The use of TNF-alpha inhibitor confirmed the ability of the CL-PMIDA-CoO nanocomplex to stimulate TNF-alpha mediated immunostimulation. CL-PMIDA-CoO nanoparticles efficiently increased the CD4(+) population."
According to the news editors, the research concluded: "Thus, our findings provide insight into the use of PMIDA coated CoO nanoparticles as antigen delivery vehicles."
For more information on this research see: Anticancer and immunostimulatory role of encapsulated tumor antigen containing cobalt oxide nanoparticles. Journal of Biological Inorganic Chemistry, 2013;18(8):957-973. Journal of Biological Inorganic Chemistry can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Journal of Biological Inorganic Chemistry - www.springerlink.com/content/0949-8257/)
Our news journalists report that additional information may be obtained by contacting S. Chattopadhyay, Indian Inst Technol, Dept. of Chem, Nano Mat Lab, Kharagpur 721302, W Bengal, India. Additional authors for this research include S.K. Dash, T. Ghosh, S. Das, S. Tripathy, D. Mandal, D. Das, P. Pramanik and S. Roy (see also Myeloid Cells).
Keywords for this news article include: Asia, India, Cobalt, Therapy, Kharagpur, Immunology, Macrophages, Nanoparticle, Myeloid Cells, Nanotechnology, Transition Elements, Emerging Technologies, Connective Tissue Cells, Mononuclear Phagocyte System
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