By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators publish new report on Biotechnology. According to news reporting originating from Shaanxi, People's Republic of China, by NewsRx correspondents, research stated, "The aim of this study was to investigate the effect of a B-cell-specific MLV integration site-1 (Bmi-1) RNA interference (RNAi) expression vector on the proliferation and invasiveness of laryngeal carcinoma. We constructed a lentiviral vector expressing Bmi-1-specific short hairpin RNA (shRNA), and transfected it into HEp-2 cells."
Our news editors obtained a quote from the research from Xi'an Jiaotong University, "Bmi-1 gene expression was detected by real-time RT-PCR and western blot analysis. We used flow cytometry and TUNEL assay to analyze the apoptosis of transfected cells, and examined cellular growth in vitro by MTT assay. We established an animal model and evaluated the therapeutic effects of small interfering RNA (siRNA) against Bmi-1. siRNA against Bmi-1 significantly knocked down Bmi-1 expression in HEp-2 cells, induced cell cycle arrest at the G1 phase, inhibited cell proliferation and promoted cell apoptosis. Lentiviral Bmi-1-shRNA vector transfection also significantly reduced cell migration. The formation and growth rate of xenograft tumors in mice transfected with siRNA against Bmi-1 was significantly reduced. The loss of mitochondrial membrane potential, the release of cytochrome c from the mitochondria into the cytosol, and the increased activity of caspase-3, -8 and -9 occurred concomitantly with the inhibition of Bmi-1."
According to the news editors, the research concluded: "Our data indicate that siRNA against Bmi-1 significantly suppresses tumor growth and induces apoptosis in vitro and in vivo."
For more information on this research see: Silencing Bmi-1 expression by RNA interference suppresses the growth of laryngeal carcinoma cells. International Journal of Molecular Medicine, 2013;31(5):1262-72 (see also technology.html">Biotechnology).
The news editors report that additional information may be obtained by contacting X.B. Yao, Dept. of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, People's Taiwan. Additional authors for this research include X.X. Wang, H. Liu, S.Q. Zhang and H.L Zhu.
Keywords for this news article include: Asia, Biotechnology, Shaanxi, Oncology, Apoptosis, Carcinoma, Laryngeal Cancer, Cancer Gene Therapy, People's Republic of China.
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