By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Biotechnology. According to news reporting originating in Chiba, Japan, by NewsRx journalists, research stated, "No licensed malaria vaccine exists, in spite of intensive development efforts. We have been investigating development of a DNA vaccine to prevent malaria infection."
The news reporters obtained a quote from the research from Ryotokuji University, "To date, we have established a full-length cDNA expression library from the erythrocytic-stage murine malaria parasite, Plasmodium berghei. We found that immunization of mice with combined 2000 clones significantly prolonged survival after challenge infection and that splenocytes from the immunized mice showed parasite-specific cytokine production. We determined the 5'-end one-pass sequence of these clones and mapped a draft genomic sequence for P. berghei for use in screening vaccine candidates for efficacy. In this study, we annotated these cDNA clones by comparing them with the genomic sequence of Plasmodium falciparum. We then divided them into several subsets based on their characteristics and examined their protective effects against malaria infection. Consequently, we selected 104 clones that strongly induced specific IgG production and decreased the mortality rate in the early phase. Most of these 104 clones coded for unknown proteins."
According to the news reporters, the research concluded: "The results suggest that these clones represent potential novel malaria vaccine candidates."
For more information on this research see: Screening of novel malaria DNA vaccine candidates using full-length cDNA library. Experimental Parasitology, 2013;135(3):546-550. Experimental Parasitology can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Experimental Parasitology - www.elsevier.com/wps/product/cws_home/622829)
Our news correspondents report that additional information may be obtained by contacting A. Shibui, Ryotokuji Univ, Fac Hlth Sci, Chiba 2798567, Japan. Additional authors for this research include S. Nakae, J. Watanabe, Y. Sato, M.E.M. Tolba, J. Doi, T. Shiibashi, S. Nogami, S. Sugano and N. Hozumi (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Chiba, Japan, DNA Research, DNA Vaccines, Synthetic Vaccines
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