By a News Reporter-Staff News Editor at Clinical Trials Week -- Current study results on DNA Research have been published. According to news originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Gold nanoparticles (AuNPs) have been reported to induce faster heat transfer of polymerase chain reaction (PCR) solution to enhance PCR efficiency. AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein (SSB)."
Our news journalists obtained a quote from the research from the Chinese Academy of Science, "However, the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity. This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures. In addition, comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy. Finally, heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities. This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity. AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers, DNA fragments and template, and enhanced PCR efficiency and specificity simultaneously."
According to the news editors, the research concluded: "Thus, this significant finding suggests the future use of AuNP-assisted PCR in different fields, especially in rapid clinical diagnosis and screening by PCR."
For more information on this research see: Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity. Chinese Science Bulletin, 2013;58(36):4593-4601. Chinese Science Bulletin can be contacted at: Science Press, 16 Donghuangchenggen North St, Beijing 100717, Peoples R China. (World Scientific Publishing - www.worldscientific.com/; Chinese Science Bulletin - www.worldscinet.com/csb/csb.shtml)
The news correspondents report that additional information may be obtained from Y. Lin, Chinese Academy Sci, Res Center Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, People's Republic of China. Additional authors for this research include J. Li, J. Yao, Y. Liang, J. Zhang, Q.F. Zhou and G.B. Jiang (see also DNA Research).
Keywords for this news article include: Asia, Beijing, DNA Research, Nanotechnology, Gold Nanoparticles, Emerging Technologies, People's Republic of China, Clinical Trials and Studies
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