By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Science. According to news reporting from Jackson, Mississippi, by NewsRx journalists, research stated, "A microchip electrophoresis-mass spectrometric (MCE-MS) method was developed for fast chiral analysis. The proposed MCE-MS platform deployed a glass/PDMS hybrid microchip with an easy-to-fabricate monolithic nanoelectrospray emitter."
The news correspondents obtained a quote from the research from the University of Mississippi, "Enantiomeric MCE separation was achieved by means of the partial filling technique. A novel chip design with an arm channel connecting to the middle of the MCE separation channel for delivering the chiral selector was tested and proven valid. Enantiomeric separation of 3.4-dihydroxyphenylalanine (DOPA), glutamic acid (Glu), and serine (Ser), the selected test compounds, were achieved within 130s with resolution values (R-s) of 2.4, 1.1, and 1.0, respectively. The proposed chiral MCE-MS assay was sensitive and had detection limits of 43 nM for L-DOPA and 47 nM for D-DOPA. The analytical platform was well suited for studies of stereochemical preference in living cells because it integrated cell culture, sample injection, chiral separation, and MS detection into a single platform. Metabolism of DOPA in human SH-SY5Y neuronal cells was studied as a model system. On-chip incubation of SH-SY5Y cells with racemic DOPA was carried out, and the incubation solution was injected and in-line assayed at time intervals. It was found that L-DOPA concentration decreased gradually as incubation time increased while the concentration of coexisting D-DOPA remained constant."
According to the news reporters, the research concluded: "The results firmly indicated that SH-SY5Y cells metabolized L-DOPA effectively while left D-DOPA intact."
For more information on this research see: A microchip electrophoresis-mass spectrometric platform for fast separation and identification of enantiomers employing the partial filling technique. Journal of Chromatography A, 2013;1318():251-256. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
Our news journalists report that additional information may be obtained by contacting X.T. Li, University of Mississippi, Medical Center, Dept. of Psychiat, Jackson, MS 39216, United States. Additional authors for this research include D. Xiao, X.M. Ou, C. McCullm and Y.M. Liu (see also Science).
Keywords for this news article include: Jackson, Science, Mississippi, United States, North and Central America
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