By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in Enzymes and Coenzymes. According to news reporting originating in Mashhad, Iran, by NewsRx journalists, research stated, "Thermal instability of the industrial enzymes such as nitrile hydratase is a major problem in biotechnology. Insolubility of hydrophobic substrate of such enzymes in aqueous solvents is another problem."
The news reporters obtained a quote from the research from Ferdowsi University, "Enzymatic reactions in hydrophobic non-aqueous solvents may be a solution for these problems. Here, using molecular dynamic simulation it is shown for the first time that hydrophobic ionic liquids can protect nitrile hydratase from thermal instability at high temperatures. Different structural properties of the enzyme were analyzed at 300, 335, and 370 K respectively. Although enzyme was partially denatured by enhancing thermal stress in water, in 1-ethyl-3-methylimidazolium bis (trifluoromethylsulfonyl) imide, as a model hydrophobic ionic liquid, enzyme conformation remained native. Ionic liquid molecules interact strongly with the surface residues of the enzyme and rigidify the structure at high temperatures. Although the spatial arrangement of the catalytic residues in the active site was destructed in hot water, the active site remained native and therefore enzyme preserved its activity in high temperature ionic liquid. In contrast to the hydrophilic enzyme products, the substrates of nitrile hydratase are commonly hydrophobic. Therefore it is noteworthy that the application of hydrophobic ionic liquids is not only recommended for their thermal stability effects on the enzyme structure, but is also a very useful way for disposability of hydrophobic substrates to the active center of the enzyme and simple isolation of the hydrophilic products from solvent."
According to the news reporters, the research concluded: "The results of this paper can propose new promising technologies for nitrile hydratase catalyzed reactions and the industrial production of important materials such as acrylamide and nicotinamide."
For more information on this research see: It is explored that ionic liquids can be suitable solvents for nitrile hydratase catalyzed reactions: A gift of the molecular modeling for the industry. Journal of Molecular Liquids, 2013;187():30-42. Journal of Molecular Liquids can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Molecular Liquids - www.elsevier.com/wps/product/cws_home/500849)
Our news correspondents report that additional information may be obtained by contacting M.R. Housaindokht, Ferdowsi Univ Mashhad, Fac Sci, Dept. of Phys, Mashhad, Iran. Additional authors for this research include H. Monhemi, H.E. Hosseini, M.S.S. Googheri, R.I. Najafabadi, N. Ashraf and M. Gholizadeh (see also Enzymes and Coenzymes).
Keywords for this news article include: Iran, Asia, Mashhad, Solvents, Ionic Liquids, Molecular Modeling, Enzymes and Coenzymes
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