By a News Reporter-Staff News Editor at Energy Weekly News -- Current study results on Gram-Positive Bacteria have been published. According to news reporting originating from Taejon, South Korea, by VerticalNews correspondents, research stated, "Staphylococcus haemolyticus L62 (SHL62) lipase was displayed on the cell surface of Escherichia coli using an autotransporter protein of Pseudomonas putida EstA beta 8 as an anchoring motif. Localization of the SHL62 lipase on the cell surface of E. coli was confirmed by immunofluorescence microscopy, flow cytometry analysis, Western blot, protease accessibility, and whole-cell enzyme activity assays."
Our news editors obtained a quote from the research from the Korea Research Institute of Chemical Technology, "SHL62 lipase activity of whole cells reached 168 U/g dry cell weight toward p-nitrophenyl caprylate after being induced by 0.1 mM IPTG for 24 h. The optimum temperature and pH of the displayed SHL62 lipase was 40-45 degrees C and pH 10, respectively. The displayed SHL62 was stable up to 45 C, at which it had >80% of maximum activity. The displayed SHL62 lipase showed a preference for medium chain fatty acids of p-nitrophenyl esters."
According to the news editors, the research concluded: "Moreover, the displayed SHL62 lipase was used as a whole cell catalyst to produce biodiesel that was obtained at a yield of nearly 89.4% after a 96 h reaction at 30 degrees C. These results suggest that the displayed SHL62 lipase on the cell surface of E. coil using an EstA beta 8 anchoring motif can be used for biocatalytic applications."
For more information on this research see: Cell surface display of Staphylococcus haemolyticus L62 lipase in Escherichia coli and its application as a whole cell biocatalyst for biodiesel production. Journal of Molecular Catalysis B-Enzymatic, 2013;97():54-61. Journal of Molecular Catalysis B-Enzymatic can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands.
The news editors report that additional information may be obtained by contacting S.J. Kim, Korea Res Inst Chem Technol, Chem Biotechnol Res Center, Taejon 305600, South Korea. Additional authors for this research include J.K. Song and H.K. Kim.
Keywords for this news article include: Asia, Biotechnology, Taejon, Energy, Lipase, Biodiesel, Bacillales, South Korea, Oil and Gas, Bioengineering, Escherichia coli, Staphylococcaceae, Enterobacteriaceae, Gram-Positive Cocci, Enzymes and Coenzymes, Gram-Negative Bacteria, Gram-Positive Bacteria, Endospore-Forming Bacteria, Carboxylic Ester Hydrolases
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