By a News Reporter-Staff News Editor at Food Weekly News -- Research findings on Electrokinetics are discussed in a new report. According to news originating from Maringa, Brazil, by VerticalNews correspondents, research stated, "The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles."
Our news journalists obtained a quote from the research from Maringa State University, "The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol L-1 borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 A degrees C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 A +/- 0.02, 6.81 A +/- 0.02, 8.13 A +/- 0.04, 8.80 A +/- 0.07, 8.98 A +/- 0.06, 11.10 A +/- 0.08, 11.34 A +/- 0.05, 13.85 A +/- 0.15, 14.82 A +/- 0.04, and 17.85 A +/- 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg L-1 and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg L-1, for vitamins PP (nicotinamide), B-12 (cyanocobalamin), B-2 (riboflavin), B-6 (pyridoxine), B-8 (biotin), C (ascorbic acid), B-5 (pantothenic acid), B-3 (nicotinic acid), B-1 (thiamine), and B-9 (folic acid), respectively."
According to the news editors, the research concluded: "Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods."
For more information on this research see: Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements. Food Analytical Methods, 2013;6(6):1592-1606. Food Analytical Methods can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Food Analytical Methods - www.springerlink.com/content/1936-9751/)
The news correspondents report that additional information may be obtained from D.C. da Silva, Maringa State University, Dept. of Quim, BR-87020900 Maringa, Parana, Brazil. Additional authors for this research include J.V. Visentainer, N.E. de Souza and C.C. Oliveira.
Keywords for this news article include: Brazil, Maringa, South America, Nanotechnology, Electrokinetics, Food Supplements, Emerging Technologies
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