By a News Reporter-Staff News Editor at Energy Weekly News -- Current study results on Cellular Structures have been published. According to news reporting originating in Ann Arbor, Michigan, by VerticalNews journalists, research stated, "Understanding in vivo drug release kinetics is critical for the development of nanoparticle-based delivery systems. In this study, we developed a fluorescence resonance energy transfer (FRET) imaging approach to noninvasively monitor in vitro and in vivo cargo release from polymeric nanoparticles."
The news reporters obtained a quote from the research from the University of Michigan, "The FRET donor dye (DiO or DiD) and acceptor dye (DiI or DiR) were individually encapsulated into poly(ethylene oxide)-b-polystyrene (PEO-PS) nanoparticles. When DiO (donor) nanoparticles and DiI (acceptor) nanoparticles were coincubated with cancer cells for 2 h, increased FRET signals were observed from cell membranes, suggesting rapid release of DiO and DiI to cell membranes. Similarly, increased FRET ratios were detected in nude mice after intravenous coadministration of DiD (donor) nanoparticles and DiR (acceptor) nanoparticles. In contrast, another group of nude mice i.v. administrated with DiD/DiR coloaded nanoparticles showed decreased FRET ratios. Based on the difference in FRET ratios between the two groups, in vivo DiD/DiR release half-life from PEO-PS nanoparticles was determined to be 9.2 mm. In addition, it was observed that the presence of cell membranes facilitated burst release of lipophilic cargos while incorporation of oleic acid-coated iron oxide into PEO-PS nanoparticles slowed the release of DiD/DiR to cell membranes."
According to the news reporters, the research concluded: "The developed in vitro and in vivo FRET imaging techniques can be used to screening stable nanoformulations for lipophilic drug delivery."
For more information on this research see: Noninvasive Fluorescence Resonance Energy Transfer Imaging of in Vivo Premature Drug Release from Polymeric Nanoparticles. Molecular Pharmaceutics, 2013;10(11):4185-4194. Molecular Pharmaceutics can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Molecular Pharmaceutics - www.pubs.acs.org/journal/mpohbp)
Our news correspondents report that additional information may be obtained by contacting P. Zou, University of Michigan, Coll Pharm, Dept. of Pharmaceut Sci, Ann Arbor, MI 48109, United States. Additional authors for this research include H.W. Chen, H.J. Paholak and D.X. Sun.
Keywords for this news article include: Michigan, Ann Arbor, Nanoparticle, United States, Cell Membrane, Nanotechnology, Cellular Structures, Emerging Technologies, North and Central America
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