By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news reporting out of Guangdong, People's Republic of China, by NewsRx editors, research stated, "We fabricated a three-layer polydimethylsiloxane (PDMS)-based microfluidic chip for, realizing urease inhibition assay with sensitive fluorescence detection. Procedures such as sample prehandling, enzyme reaction, reagent mixing, fluorescence derivatization, and detection can be readily carried out."
Our news journalists obtained a quote from the research from Sun Yat Sen University, "Urease reactors were prepared by adsorption of rabbit immunoglobulin G (IgG) and immunoreaction with urease-conjugated goat anti-rabbit IgG. Acetohydroxamic acid (AHA) as a competitive inhibitor of urease was tested on the chip. Microfluidically generated gradient concentrations of AHA with substrate (urea) were loaded into urease reactors. After incubation, the produced ammonia was transported out of reactors and then reacted with o-phthalaldehyde (OPA) to generate fluorescent products. Urease inhibition was indicated by a decrease in fluorescence signal detected by microplate reader. The IC50 value of AHA was determined and showed good agreement with that obtained in microplate. The presented device combines several steps of the analytical process with advantages of low reagent consumption, reduced analysis time, and ease of manipulation."
According to the news editors, the research concluded: "This microfluidic approach can be extended to the screening of inhibitory compounds in drug discovery."
For more information on this research see: Fluorimetric urease inhibition assay on a multilayer microfluidic chip with immunoaffinity immobilized enzyme reactors. Analytical Biochemistry, 2013;441(1):51-57. Analytical Biochemistry can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Analytical Biochemistry - www.elsevier.com/wps/product/cws_home/622781)
Our news journalists report that additional information may be obtained by contacting Q. Zhang, Sun Yat Sen UniversitySch Pharmaceut Sci, Guangzhou 510006, Guangdong, People's Republic of China. Additional authors for this research include X.W. Tang, F.H. Hou, J.P. Yang, Z.Y. Xie and Z.Y. Cheng (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Urease, Guangdong, Amidohydrolases, Immobilized Enzymes, Immobilized Proteins, Enzymes and Coenzymes, People's Republic of China
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