By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Biotechnology have been presented. According to news reporting originating in Jinan, People's Republic of China, by NewsRx journalists, research stated, "Toxoplasma gondii is an obligate intracellular parasite that can pose a serious threat to human health by causing toxoplasmosis. There are no drugs that target the chronic cyst stage of this infection; therefore, development of an effective vaccine would be an important advance."
The news reporters obtained a quote from the research from the Shandong University School of Medicine, "Aspartic proteases play essential roles in the T. gondii lifecycle. The parasite has four aspartic protease encoding genes, which are called toxomepsin 1, 2, 3 and 5 (TgASP1, 2, 3 and 5, respectively). Bioinformatics approaches have enabled us to identify several promising linear-B cell epitopes and potential Th-cell epitopes on TgASP1, thus supporting its potential as a DNA vaccine against toxoplasmosis. We expressed TgASP1 in Escherichia coli and used the purified protein to immunize BALB/c mice. The antibodies obtained were used to determine where TgASP1 was localized in the parasite. We also made a TgASP1 DNA vaccine construct and evaluated it for the level of protection conferred to mice against infection with the virulent RH strain of T. gondii. TgASP1 appears to be a membrane protein located primarily at the tip of the T. gondii tachyzoite. Investigation of its potential as a DNA vaccine showed that it elicited strong humoral and cellular immune responses in mice, and that these responses were mediated by Th-1 cells. Mice immunized with the vaccine had greater levels of protection against mortality following challenge with T. gondii RH tachyzoites than did those immunized with PBS or the empty vector control."
According to the news reporters, the research concluded: "TgASP1 is a novel candidate DNA vaccine that merits further investigation."
For more information on this research see: Identification and characterization of Toxoplasma gondii aspartic protease 1 as a novel vaccine candidate against toxoplasmosis. Parasites & Vectors, 2013;6():175. (BioMed Central - www.biomedcentral.com/; Parasites & Vectors - www.parasitesandvectors.com)
Our news correspondents report that additional information may be obtained by contacting G. Zhao, Dept. of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province, People's Taiwan. Additional authors for this research include A. Zhou, G. Lu, M. Meng, M. Sun, Y. Bai, Y. Han, L. Wang, H. Zhou, H. Cong, Q. Zhao, X.Q. Zhu and S. He (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Jinan, DNA Research, DNA Vaccines, Synthetic Vaccines, People's Republic of China.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC