By a News Reporter-Staff News Editor at Drug Week -- New research on Sulfur Amino Acids is the subject of a report. According to news reporting out of Shanghai, People's Republic of China, by NewsRx editors, research stated, "The lack of effective delivery vehicles impedes in vivo applications of siRNA. The trimethyl chitosan-cysteine (TC) nanoparticles (NPs) were developed for in vivo delivery of tumor necrosis factor alpha (TNF-alpha) siRNA via oral gavage and intraperitoneal injection."
Our news journalists obtained a quote from the research from Fudan University, "The nanoparticles formulated from TC conjugate of 100, 200, and 500 kDa were prepared through ionic gelation with sodium tripolyphosphate, termed as TC100 NPs, TC200 NPs, and TC500 NPs, respectively. They were evaluated in terms of stability, siRNA protection, cellular uptake and TNF-alpha knockdown in peritoneal exudates macrophage cells (PECs), and in vivo TNF-alpha silencing in acute hepatic injury mice. TC100 NPs exhibited poor stability in simulated physiological environment compared to TC200 NPs and TC500 NPs. Compared to TC500 NPs, TC200 NPs could significantly enhance in vitro and in vivo cellular uptake by PECs and facilitate cytoplasmic siRNA release, resulting in high in vitro and in vivo TNF-alpha knockdown. Superior TNF-alpha suppressing level was obtained with TC200 NPs via oral gavage rather than intraperitoneal injection."
According to the news editors, the research concluded: "The efficacies of in vivo TNF-alpha silencing were related to the molecular weight of TC conjugate and the administration route, which would assist in the rational design of siRNA vehicles."
For more information on this research see: Trimethyl Chitosan-Cysteine Nanoparticles for Systemic Delivery of TNF-alpha siRNA via Oral and Intraperitoneal Routes. Pharmaceutical Research, 2013;30(10):2596-2606. Pharmaceutical Research can be contacted at: Springer, Plenum Publishers, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Pharmaceutical Research - www.springerlink.com/content/0724-8741/)
Our news journalists report that additional information may be obtained by contacting C.B. He, Fudan University, Sch Life Sci, Dept. of Pharmaceut Sci, State Key Lab Genet Engn, Shanghai 200433, People's Republic of China. Additional authors for this research include L.C. Yin, C. Tang and C.H. Yin (see also Sulfur Amino Acids).
Keywords for this news article include: Asia, Shanghai, Cysteine, Nanoparticle, Nanotechnology, Sulfur Amino Acids, Neutral Amino Acids, Sulfhydryl Compounds, Emerging Technologies, People's Republic of China
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