By a News Reporter-Staff News Editor at Cancer Weekly -- Research findings on Oncology are discussed in a new report. According to news reporting from Leipzig, Germany, by NewsRx journalists, research stated, "The provirus integration site for Moloney murine leukemia virus 1 (Pim-1) kinase is overexpressed in various tumors and has been linked to poor prognosis. Its role as proto-oncogene is based on several Pim-1 target proteins involved in pivotal cellular processes."
The news correspondents obtained a quote from the research from the University of Leipzig, "Here, we explore the functional relevance of Pim-1 in colon carcinoma. RNAi-based knockdown approaches, as well as a specific small molecule inhibitor, were used to inhibit Pim-1 in colon carcinoma cells. The effects were analyzed regarding proliferation, apoptosis, sensitization toward cytostatic treatment, and overall antitumor effect in vitro and in mouse tumor models in vivo. We demonstrate antiproliferative, proapoptotic, and overall antitumor effects of Pim-1 inhibition. The sensitization to 5-fluorouracil (5-FU) treatment upon Pim-1 knockdown offers new possibilities for combinatorial treatment approaches. Importantly, this also antagonizes a 5-FU-triggered Pim-1 up-regulation, which is mediated by decreased levels of miR-15b, a microRNA we newly identify to regulate Pim-1. The analysis of the molecular effects of Pim-1 inhibition reveals a complex regulatory network, with therapeutic Pim-1 repression leading to major changes in oncogenic signal transduction with regard to p21(Cip1/WAF1), STAT3, c-jun-N-terminal kinase (JNK), c-Myc, and survivin and in the levels of apoptosis-related proteins Puma, Bax, and Bcl-xL. We demonstrate that Pim-1 plays a pivotal role in several tumor-relevant signaling pathways and establish the functional relevance of Pim-1 in colon carcinoma."
According to the news reporters, the research concluded: "Our results also substantiate the RNAi-mediated Pim-1 knockdown based on polymeric polyethylenimine/small interfering RNA nanoparticles as a promising therapeutic approach."
For more information on this research see: Functional role and therapeutic potential of the pim-1 kinase in colon carcinoma. Neoplasia, 2013;15(7):783-94 (see also Oncology).
Our news journalists report that additional information may be obtained by contacting U. Weirauch, Rudolf Boehm Institute for Pharmacology and Toxicology, Clinical Pharmacology, University of Leipzig, Leipzig, Germany. Additional authors for this research include N. Beckmann, M. Thomas, A. Grunweller, K. Huber, F. Bracher, R.K. Hartmann and A. Aigner.
Keywords for this news article include: Europe, Kinase, Leipzig, Germany, Genetics, Oncology, Carcinoma, Treatment, Colon Cancer, Gastroenterology, Enzymes and Coenzymes.
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