By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Researchers detail new data in Biotechnology. According to news reporting from Mashhad, Iran, by NewsRx journalists, research stated, "Functionalized carbon nanotubes (CNTs) have been recently emerged as important class of vectors for delivery of DNA and other biomolecules into various cells. In this study, single-walled carbon nanotubes (SWNTs) were functionalized by non-covalent binding of hydrophobic moieties, which were covalently linked to polyethyleneimines (PEIs)."
The news correspondents obtained a quote from the research from the Mashhad University of Medical Sciences, "PEls of three molecular weights (25, 10 and 1.8 kDa) were used. CNTs were functionalized with the PEI series either through phospholipid moiety (via a polyethyleneglycol linker) or through directly-attached long (18 carbons) or intermediate (10 carbons) hydrophobic alkyl moieties. All PEI-functionalized CNTs exhibited good stability and dispersibility in biological media. Visualizing of functionalized CNTs and lack of aggregation were confirmed by atomic force microscopy. The PEI derivatives bound to CNTs retained the ability to fully condense plasmid DNA at low NIP ratios and substantial buffering capacity in the endosomal pH range. PEI-functionalized CNTs exhibited increased transfection efficiency compared to underivatized PEIs up to 19-fold increase being observed in the functionalized CNT with the smallest PEI tested, the smallest hydrophobic attachment moiety tested and no linker."
According to the news reporters, the research concluded: "Also PEI-functionalized CNTs were effective gene delivery vectors in vivo following tail vein injection in mice with the largest expression occurring with the vector PEI-functionalized through a polyethyleneglycol linker."
For more information on this research see: Non-covalent functionalization of single-walled carbon nanotubes with modified polyethyleneimines for efficient gene delivery. International Journal of Pharmaceutics, 2013;454(1):204-215. International Journal of Pharmaceutics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; International Journal of Pharmaceutics - www.elsevier.com/wps/product/cws_home/505513)
Our news journalists report that additional information may be obtained by contacting B. Behnam, Mashhad Univ Med Sci, Sch Pharm, Nanotechnol Res Center, Mashhad, Iran. Additional authors for this research include W.T. Shier, A.H. Nia, K. Abnous and M. Ramezani (see also technology.html">Biotechnology).
Keywords for this news article include: Iran, Asia, Biotechnology, Mashhad, Alkenes, Polyenes, Fullerenes, Gene Therapy, Hydrocarbons, Bioengineering, Carbon Nanotubes, Organic Chemicals, Polyethyleneimine
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC