By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting originating in Chongqing, People's Republic of China, by NewsRx journalists, research stated, "Two recombinant Rhizopus stolonifer a dagger 6-fatty acid desaturase enzymes with different-length N-termini were cloned and expressed in Saccharomyces cerevisiae strain INVScl: LRsD6D begins with the sequence of the N-terminal of the R. stolonifer a dagger 6-fatty acid desaturase native, encoding a deduced polypeptide of 459 amino acids (M-S-T-L-D-R-Q-S-I-F-T-I-K-E-L-E-S-I-S-Q-R-I-H-D-G-D-E-E-A-M-K-F), whereas SRsD6D begins with the amino acid sequence of the predicted ORF, encoding a deduced polypeptide of 430 amino acids (M-K-F) and LRsD6D is longer than SRsD6D by 29 amino acids (M-S-T-L-D-R-Q-S-I-F-T-I-K-E-L-E-S-I-S-Q-R-I-H-D-G-D-E-E-A)."
The news reporters obtained a quote from the research from the Chongqing University of Medical Sciences, "Bioinformatic analysis characterized the two recombinant a dagger 6-fatty acid desaturase enzymes with different-length N-termini, including three conserved histidine-rich motifs, hydropathy profile, and a cytochrome b5-like domain in the N-terminus. When the coding sequence was expressed in S. cerevisiae strain INVScl, the coding produced a dagger 6-fatty acid desaturase activity exhibited by RsD6D, leading to a novel peak corresponding to gamma-linolenic acid methyl ester standards, which was detected with the same retention time."
According to the news reporters, the research concluded: "The residual activity of LRsD6D was 74 % at 15 A degrees C for 4 h and that of SRsD6D was 43 %. Purified recombinant LRsD6D was more stable than SRsD6D, indicating that the N-terminal extension, containing mostly hydrophobic residues, affected the overall stability of recombinant LRsD6D."
For more information on this research see: The Thermostability of Two Kinds of Recombinant Delta 6-Fatty Acid Desaturase with Different N-Terminal Sequence Lengths in Low Temperature. Applied Biochemistry and Biotechnology, 2013;171(1):165-172. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)
Our news correspondents report that additional information may be obtained by contacting H. Lu, Chongqing Univ Med Sci, Dept. of Immunol, Chongqing 400016, People's Republic of China (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Peptides, Proteins, Chongqing, Amino Acids, Oxidoreductases, Enzymes and Coenzymes, Fatty Acid Desaturases, Mixed Function Oxygenases, People's Republic of China
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