By a News Reporter-Staff News Editor at Gene Therapy Weekly -- New research on Retinal Neurons is the subject of a report. According to news originating from Detroit, Michigan, by NewsRx correspondents, research stated, "Expression of optogenetic tools in surviving inner retinal neurons to impart retinal light sensitivity has been a new strategy for restoring vision after photoreceptor degeneration. One potential approach for restoring retinal light sensitivity after photoreceptor degeneration is to express optogenetic tools in retinal ganglion cells (RGCs)."
Our news journalists obtained a quote from the research from the Wayne State University School of Medicine, "For this approach, restoration of ON and OFF center-surround receptive fields in RGCs, a key feature of visual information processing, may be important. A possible solution is to differentially express depolarizing and hyperpolarizing optogenetic tools, such as channelrhodopsin-2 and halorhodopsin, to the center and peripheral regions of the RGC dendritic field by using protein targeting motifs. Recombinant adeno-associated virus (rAAV) vectors have proven to be a powerful vehicle for in vitro and in vivo gene delivery, including in the retina. Therefore, the search for protein targeting motifs that can achieve rAAV-mediated subcellular targeted expression would be particularly valuable for developing therapeutic applications. In this study, we identified two protein motifs that are suitable for rAAV-mediated subcellular targeting for generating center-surround receptive fields while reducing the axonal expression in RGCs."
According to the news editors, the research concluded: "Resulting morphological dendritic field and physiological response field by center-targeting were significantly smaller than those produced by surround-targeting. rAAV motif-mediated protein targeting could also be a valuable tool for studying physiological function and clinical applications in other areas of the central nervous system."
For more information on this research see: rAAV-mediated subcellular targeting of optogenetic tools in retinal ganglion cells in vivo. Plos One, 2013;8(6):e66332. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news correspondents report that additional information may be obtained from C. Wu, Dept. of Anatomy, Cell Biology, Wayne State University School of Medicine, Detroit, Michigan, United States. Additional authors for this research include E. Ivanova, Y. Zhang and Z.H Pan (see also Retinal Neurons).
Keywords for this news article include: Biotechnology, Detroit, Michigan, Gene Therapy, United States, Bioengineering, Retinal Neurons, Retinal Ganglion Cells, North and Central America.
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