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By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Analytical Science. According to news reporting out of Beijing, People's Republic of China, by NewsRx editors, research stated, "As an edible and medicinal plant, Coix seed is readily contaminated by more than one group of mycotoxins resulting in potential risk to human health. A reliable and sensitive method has been developed to determine seven mycotoxins (aflatoxins B-1, B-2, G(1), G(2), zearalenone, alpha-zearalenol, and beta-zearalenol) simultaneously in 10 batches of Coix seed marketed in China."
Our news journalists obtained a quote from the research from Center for Biotechnology, "The method is based on a rapid ultrasound-assisted solid-liquid extraction (USLE) using methanol/water (80/20) followed by immunoaffinity column (IAC) clean-up, on-line photochemical derivatization (PCD), and high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD). Careful optimization of extraction, clean-up, separation and detection conditions was accomplished to increase sample throughput and to attain rapid separation and sensitive detection. Method validation was performed by analyzing samples spiked at three different concentrations for the seven mycotoxins. Recoveries were from 73.5% to 107.3%, with relative standard deviations (RSDs) lower than 7.7%. The intra-and inter-day precisions, expressed as RSDs, were lower than 4% for all studied analytes. Limits of detection and quantification ranged from 0.01 to 50.2 mu g kg(-1), and from 0.04 to 125.5 mu g kg(-1), respectively, which were below the tolerance levels for mycotoxins set by the European Union. Samples that tested positive were further analyzed by HPLC tandem electrospray ionization mass spectrometry for confirmatory purposes."
According to the news editors, the research concluded: "This is the first application of USLE-IAC-HPLC-PCD-FLD for detecting the occurrence of multi-class mycotoxins in Coix seed."
For more information on this research see: Development of a sensitive and reliable high performance liquid chromatography method with fluorescence detection for high-throughput analysis of multi-class mycotoxins in Coix seed. Analytica Chimica Acta, 2013;799():68-76. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
Our news journalists report that additional information may be obtained by contacting W.J. Kong, China Natl Center Biotechnol Dev, Beijing 100081, People's Republic of China. Additional authors for this research include J.Y. Li, F. Qiu, J.H. Wei, X.H. Xiao, Y.G. Zheng and M.H. Yang (see also Analytical Science).
Keywords for this news article include: Asia, Beijing, Analytical Science, Imaging Technology, People's Republic of China, High-Performance Liquid Chromatography
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