technology Have Reported New Data on Glycoside Hydrolases -->
By a News Reporter-Staff News Editor at Science Letter -- Researchers detail new data in Enzymes and Coenzymes. According to news reporting originating from New Delhi, India, by NewsRx correspondents, research stated, "Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase gene expression varies from high to negligible."
Our news editors obtained a quote from the research from International Center for Genetic Engineering and Biotechnology, "In the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. MicroRNA library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several miRNAs. In silico analysis of sequenced miRNAs revealed three miRNAs having sequence similarity to 3'UTR of chitinase. Gene-targeted specific action of these miRNAs, was investigated by luciferase reporter having 3'UTR of chitinase. Only one of three miRNAs, miR-24, inhibited luciferase expression. Further, a day-wise in vivo quantification of miR-24 in fifth-instar larvae revealed a negative correlation with corresponding chitinase transcript abundance. The force-feeding of synthetic miR-24 induced significant morphological aberrations accompanied with arrest of molting."
According to the news editors, the research concluded: "These miR-24 force-fed larvae revealed significantly reduced chitinase transcript abundance."
For more information on this research see: Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA. Scientific Reports, 2013;3():2292. (Nature Publishing Group - www.nature.com/; Scientific Reports - www.nature.com/srep/)
The news editors report that additional information may be obtained by contacting N. Agrawal, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, PO Box 10504, New Delhi-110067, India. Additional authors for this research include B. Sachdev, J. Rodrigues, K.S. Sree and R.K Bhatnagar (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, New Delhi, Chitinase, Glycoside Hydrolases, Enzymes and Coenzymes.
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