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By a News Reporter-Staff News Editor at Science Letter -- Investigators discuss new findings in Cell Science. According to news reporting originating in Pisa, Italy, by NewsRx journalists, research stated, "The neurotrophin receptor TrkA (also known as NTRK1) is known to be crucially involved in several physio-pathological processes. However, a clear description of the early steps of ligand-induced TrkA responses at the cell plasma membrane is missing."
The news reporters obtained a quote from the research from Center for Nanotechnology, "We have exploited single particle tracking and TIRF microscopy to study TrkA membrane lateral mobility and changes of oligomerization state upon binding of diverse TrkA agonists (NGF, NGF R100E HSANV mutant, proNGF and NT-3). We show that, in the absence of ligands, most of the TrkA receptors are fast moving monomers characterized by an average diffusion coefficient of 0.47 mu m(2)/second; about 20% of TrkA molecules move at least an order of magnitude slower and around 4% are almost immobile within regions of about 0.6 mu m diameter. Ligand binding results in increased slow and/or immobile populations over the fast one, slowing down of non-immobile trajectories and reduction of confinement areas, observations that are consistent with the formation of receptor dimeric and oligomeric states. We demonstrate that the extent of TrkA lateral mobility modification is strictly ligand dependent and that each ligand promotes distinct trajectory patterns of TrkA receptors at the cell membrane (ligand 'fingerprinting' effect). This ligand signature of receptor dynamics results from a differential combination of receptor-binding affinity, intracellular effectors recruited in the signalling platforms and formation of signalling and/or recycling endosome precursors."
According to the news reporters, the research concluded: "Thus, our data uncover a close correlation between the initial receptor membrane dynamics triggered upon binding and the specific biological outcomes induced by different ligands for the same receptor."
For more information on this research see: Ligand signature in the membrane dynamics of single TrkA receptor molecules. Journal of Cell Science, 2013;126(19):4445-4456. Journal of Cell Science can be contacted at: Company Of Biologists Ltd, Bidder Building Cambridge Commercial Park Cowley Rd, Cambridge CB4 4DL, Cambs, England (see also Cell Science).
Our news correspondents report that additional information may be obtained by contacting L. Marchetti, Center Nanotechnol Innovat, IIT NEST, I-56126 Pisa, Italy. Additional authors for this research include A. Callegari, S. Luin, G. Signore, A. Viegi, F. Beltram and A. Cattaneo.
Keywords for this news article include: Pisa, Italy, Europe, Cell Science
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