By a News Reporter-Staff News Editor at Science Letter -- A new study on Chromatography is now available. According to news reporting originating in Krakow, Poland, by NewsRx journalists, research stated, "A complex extraction system with the use of cloud-point extraction technique (CPE) was developed for sequential isolation of basic and acidic/neutral medicaments from human plasma/serum, screened by HPLC/DAD method. Eight model drugs (paracetamol, promazine, chlorpromazine, amitriptyline, salicyclic acid, opipramol, alprazolam and carbamazepine) were chosen for the study of optimal CPE conditions."
The news reporters obtained a quote from the research from Jagiellonian University, "The CPE technique consists in partition of an aqueous sample with addition of a surfactant into two phases: micelle-rich phase with the isolated compounds and water phase containing a surfactant below the critical micellar concentration, mainly under influence of temperature change. The proposed extraction system consists of two chief steps: isolation of basic compounds (from pH 12) and then isolation of acidic/neutral compounds (from pH 6) using surfactant Triton X-114 as the extraction medium. Extraction recovery varied from 25.2 to 107.9% with intra-day and inter-day precision (RSD %) ranged 0.88-1087 and 5.32-17.96, respectively. The limits of detection for the studied medicaments at lambda 254 nm corresponded to therapeutic or low toxic plasma concentration levels."
According to the news reporters, the research concluded: "Usefulness of the proposed CPE-HPLC/DAD method for toxicological drug screening was tested via its application to analysis of two serum samples taken from patients suspected of drug overdosing. ."
For more information on this research see: Sequential cloud-point extraction for toxicological screening analysis of medicaments in human plasma by high pressure liquid chromatography with diode array detector. Journal of Chromatography A, 2013;1312():42-48. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)
Our news correspondents report that additional information may be obtained by contacting K. Madej, Jagiellonian University, Coll Med, Lab Analyt Toxicol & Drug Monitoring, PL-31501 Krakow, Poland. Additional authors for this research include K. Persona, M. Wandas and E. Gomolka (see also Chromatography).
Keywords for this news article include: Krakow, Poland, Europe, Therapeutics, Chromatography
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