By a News Reporter-Staff News Editor at Health & Medicine Week -- Current study results on Gram-Negative Bacterial Infections have been published. According to news reporting originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "A sensitive and efficient electrochemical immunosensor was designed for amperometric detection of heat-killed Escherichia coli O157:H7 (E. coli O157:H7). The immunosensing platform was first composed of fullerene (C60), ferrocene (Fc) and thiolated chitosan (CHI-SH) composite nano-layer which could offer rich -SH functional groups and maintain good biocompatibility."
Our news editors obtained a quote from the research from Third Military Medical University, "Then the Au nanoparticles coated SiO2 nanocomposites (Au-SiO2) were assembled on the CHI-SH/Fc/C60 composite. Next, the large amount of avidin (SA) was coated on the Au-SiO2 surface, which was used to immobilize biotinylated capture antibodies of E. coli O157:H7 (bio-Ab1) through the covalent reaction between biotin and avidin. With surface area enhancement by C60 and Au-SiO2, and directional immobilization by avidin-biotin system, the amount of immobilized bio-Ab1 can be enhanced obviously. For signal amplification, the glucose oxidase (GOD) loaded Pt nanochains (PtNCs) were used as tracing tag to label signal antibodies (Ab2). With a sandwich-type immunoreaction, the concentration volume of heat-killed E. coli O157:H7 ranged from 3.2 x 10(1) to 3.2 x 106CFU/mL with a limit of detection down to 15 CFU/mL (S/N=3), which could be well accepted for early clinical detection."
According to the news editors, the research concluded: "The studied system provides new opportunities, and might speed up disease diagnosis, treatment and prevention with pathogen."
For more information on this research see: An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag. Biosensors & Bioelectronics, 2013;49():485-91. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting Y. Li, Dept. of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, People's Taiwan. Additional authors for this research include L. Fang, P. Cheng, J. Deng, L. Jiang, H. Huang and J. Zheng (see also Gram-Negative Bacterial Infections).
Keywords for this news article include: Asia, Antibodies, Avidin, Chongqing, Chemistry, Ovalbumin, Immunology, Glycoproteins, Blood Proteins, Electrochemical, Immunoglobulins, Dietary Proteins, Gammaproteobacteria, Escherichia coli O157, People's Republic of China, Escherichia coli Infections, Enterobacteriaceae Infections, Shiga Toxigenic Escherichia coli.
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